Bladder cancer (BCa) is a heterogeneous disease that poses great threats on public health. Increasing studies have identified the vital functions of circular RNAs (circRNAs) in BCa treatment. Hence, this current study set out to explore the modulatory role of circ_0001495 in BCa development. First, the expression of circ_0001495 was determined by reverse transcription quantitative polymerase chain reaction. Cell biological processes were then analyzed after altering the circ_0001495 expression in T24 cells. Next, interactions among circ_0001495, microRNA-527 (miR-527) and roundabout guidance receptor 1 (Robo1) were investigated by dual luciferase reporter gene assay, RNA pull down assay and FISH assay. Lastly, xenograft tumors in nude mice were established to explore the effect of circ_0001495 in vivo. It was found that circ_0001495 was highly expressed in BCa tissues and cells, and was further correlated with poor prognosis in BCa patients. In addition, circ_0001495 inhibited the activity of miR-527 by acting as a sponge to sponge miR-527, which further elevated the Robo1 expression. Lastly, circ_0001495 was found to promote the proliferation, migration and invasion of BCa cells in vitro through the miR-527/Robo1 axis and promote the growth and metastasis of BCa tumors in vivo. Altogether, findings in our study highlight the promoting role of circ_0001495 in the progression of BCa by increasing Robo1 via sponging miR-527, representing a promising target for BCa management.

中文翻译：

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环状 RNA_0001495 通过海绵状 microRNA-527 增加 Robo1 表达，促进膀胱癌细胞的增殖、迁移和侵袭。

膀胱癌（BCa）是一种异质性疾病，对公众健康构成巨大威胁。越来越多的研究已经确定了环状 RNA（circRNA）在 BCa 治疗中的重要功能。因此，本研究旨在探索 circ_0001495 在 BCa 发育中的调节作用。首先，通过逆转录定量聚合酶链反应确定circ_0001495的表达。然后在改变 T24 细胞中的 circ_0001495 表达后分析细胞生物学过程。接下来，通过双荧光素酶报告基因测定、RNA 下拉测定和 FISH 测定研究了 circ_0001495、microRNA-527 (miR-527) 和环形引导受体 1 (Robo1) 之间的相互作用。最后，在裸鼠中建立异种移植肿瘤以探索circ_0001495在体内的作用。发现circ_0001495在BCa组织和细胞中高表达，进一步与BCa患者预后不良相关。此外，circ_0001495通过充当海绵来海绵miR-527来抑制miR-527的活性，这进一步提高了Robo1的表达。最后，发现circ_0001495通过miR-527/Robo1轴在体外促进BCa细胞增殖、迁移和侵袭，在体内促进BCa肿瘤的生长和转移。总而言之，我们的研究结果强调了 circ_0001495 通过海绵 miR-527 增加 Robo1 在 BCa 进展中的促进作用，这是 BCa 管理的一个有希望的目标。circ_0001495 通过充当海绵来海绵 miR-527 来抑制 miR-527 的活性，这进一步提高了 Robo1 的表达。最后，发现circ_0001495通过miR-527/Robo1轴在体外促进BCa细胞增殖、迁移和侵袭，在体内促进BCa肿瘤的生长和转移。总而言之，我们的研究结果强调了 circ_0001495 通过海绵 miR-527 增加 Robo1 在 BCa 进展中的促进作用，这是 BCa 管理的一个有希望的目标。circ_0001495 通过充当海绵来海绵 miR-527 来抑制 miR-527 的活性，这进一步提高了 Robo1 的表达。最后，发现circ_0001495通过miR-527/Robo1轴在体外促进BCa细胞增殖、迁移和侵袭，在体内促进BCa肿瘤的生长和转移。总而言之，我们的研究结果强调了 circ_0001495 通过海绵 miR-527 增加 Robo1 在 BCa 进展中的促进作用，这是 BCa 管理的一个有希望的目标。