Antithrombin (AT) is a glycoprotein produced by the liver and a principal antagonist of active clotting proteases. A deficit in AT function leads to AT qualitative deficiency, challenging to diagnose. Here we report that active AT may travel physiosorbed on the surface of plasma extracellular vesicles (EVs), contributing to form the “EV-protein corona”. The corona is enriched in specific AT glycoforms, thus suggesting glycosylation to play a key role in AT partitioning between EVs and plasma. Differences in AT glycoform composition of the corona of EVs separated from plasma of healthy and AT qualitative deficiency-affected subjects were also noticed. This suggests deconstructing the plasma into its nanostructured components, as extracellular vesicles, could help to unravel pathophysiological mechanisms otherwise undiscovered.

中文翻译：

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活性抗凝血酶糖型选择性地物理吸附在血浆细胞外囊泡上

抗凝血酶 (AT) 是一种由肝脏产生的糖蛋白，是活性凝血蛋白酶的主要拮抗剂。AT 功能缺陷导致 AT 定性缺陷，难以诊断。在这里，我们报告说，活性 AT 可能会在血浆细胞外囊泡 (EV) 的表面物理吸附，从而形成“EV 蛋白冠”。电晕富含特定的 AT 糖型，因此表明糖基化在 EV 和血浆之间的 AT 分配中起关键作用。还注意到从健康和受 AT 定性缺陷影响的受试者血浆中分离的 EV 电晕的 AT 糖型组成的差异。这表明将血浆解构成其纳米结构成分，作为细胞外囊泡，可以帮助解开其他未被发现的病理生理机制。