Phytophthora root and stem rot is a major constraint to soybean [Glycine max (L.) Merr.] production worldwide. Deployment of single dominant Resistance to Phytophthora sojae (Rps) genes are an effective management strategy for this disease. However, due to increasing diversity in P. sojae populations for pathotype, new effective Rps genes are needed. Two recombinant inbred line (RIL) populations, each derived from a cross with Williams (susceptible) and resistant accessions PI 407974B and PI 424487B, were evaluated for resistance with one or more P. sojae pathotypes: OH1 (vir 7), OH4 (vir 1a, 1c, 7), OH7 (vir 1a, 3a, 3c, 4, 5, 6, 7), OH25 (vir 1a, 1b, 1c, 1k, 7), and 1.S.1.1 (1a, 1b, 1k, 2, 3a, 3c, 4, 5, 6, 7, 8). Molecular maps were assembled with BARCSoySNP6K BeadChip, simple-sequence repeat, and Kompetitive Allele Specific polymerase chain reaction markers. A total of three Rps loci were mapped, one near Rps1 on Chromosome 3 and two near Rps4/6 on chromosome 18. Quantitative trait loci and straight linkage maps confirmed the loci. Resistance to P. sojae pathotypes 1.S.1.1 and/or OH7 was mapped to Chromosome 3 in the PI 407974B RIL population. PI 407974B and PI 424487B RIL populations have Rps loci on chromosome 18 toward OH4 and OH25, respectively, which are near the Rps4/6 region. Although these PIs may have novel Rps genes/alleles and could assist in the deployment and pyramiding of resistance against P. sojae, care should be taken because these may condition defense reactions to some P. sojae pathotypes but not to all.

中文翻译：

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韩国大豆植物引种 407974B 和 424487B 中大豆疫霉抗性位点的鉴定

疫霉根和茎腐病是全球大豆 [ Glycine max (L.) Merr.] 生产的主要限制因素。部署单一显性抗大豆疫霉( Rps ) 基因是该病害的有效管理策略。然而，由于酱油菌群致病型的多样性增加，需要新的有效Rps基因。两个重组近交系 (RIL) 群体，每个来自与威廉姆斯（易感）和抗性种质 PI 407974B 和 PI 424487B 的杂交，对一种或多种大豆大豆球菌的抗性进行了评估致病型：OH1 (vir 7), OH4 (vir 1a, 1c, 7), OH7 (vir 1a, 3a, 3c, 4, 5, 6, 7), OH25 (vir 1a, 1b, 1c, 1k, 7),和 1.S.1.1（1a、1b、1k、2、3a、3c、4、5、6、7、8）。使用 BARCSoySNP6K BeadChip、简单序列重复和竞争性等位基因特异性聚合酶链反应标记物组装分子图。共绘制了三个Rps基因座，一个靠近染色体 3 上的Rps1，两个靠近染色体 18 上的Rps4/6。数量性状基因座和直连锁图证实了该基因座。在 PI 407974B RIL 群体中，对酱油病原菌 1.S.1.1 和/或 OH7 的抗性被定位到染色体 3 。PI 407974B 和 PI 424487B RIL 群体具有Rps18 号染色体上的基因座分别朝向 OH4 和 OH25，它们靠近Rps4/6区域。尽管这些 PIs 可能具有新的Rps基因/等位基因，并且可以帮助部署和加强对酱油假单胞菌的抗性，但应小心，因为这些可能会影响对某些酱油假单胞菌致病型的防御反应，但并非对所有病原体都产生影响。