The present study envisaged the stoichiometry of microbial biomass in petroleum-contaminated soil, in order to study the influence of the petroleum-contaminated soil on the ecosystem stability. A typical oil well area in the Northern Shaanxi was considered the research object and the oil pollution status was assessed by studying the physical, chemical, and microbiological characteristics of the soil in the area. From the measurement and analysis of the petroleum pollutants in the soil samples, it was observed that the concentration of the petroleum pollutants around all the oil well areas was higher than the critical value of 500 mg/kg. Furthermore, the C to N ratio of 8 soil samples around the oil wells (0.8:1~13.3:1) was lower than that of the control soil samples in most cases and could not reach the nutrient proportion level required by soil microorganisms. It was observed that the oil organic carbon content at 0~10 m from the wellhead was obviously higher than that in other areas, and decreased with an increase in the distance from the well. Based on the determination of soil organic carbon, total nitrogen, total phosphorus, and the soil microbial biomass carbon (SMBC), soil microbial biomass nitrogen (SMBN), and phosphorus content analysis, it was observed that only the soil organic carbon was significantly positively correlated to the oil pollutants in soil. Imbalance in the C to N, SMBC, and SMBN ratio can lead to an acute shortage of the required nutrients than microorganisms, limit the soil microbial reproduction and growth, and thereby slow down the rate of indigenous microbial degradation of petroleum hydrocarbons, so as to reduce the impact of oil pollution on the stability of the entire ecosystem. Therefore, during the remediation of petroleum-contaminated soil in this study area, adequate nutrients need to be reasonably added to the soil.

中文翻译：

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黄土丘陵区石油污染土壤微生物量的化学计量特征

本研究设想石油污染土壤中微生物生物量的化学计量，以研究石油污染土壤对生态系统稳定性的影响。以陕北典型油井区为研究对象，通过研究该地区土壤的物理、化学和微生物特征，对油​​田污染状况进行评估。通过对土壤样品中石油类污染物的测定和分析，观察到所有油井区周围石油类污染物的浓度均高于临界值500 mg/kg。此外，油井周围8个土样的碳氮比（0.8:1~13.3: 1）在大多数情况下低于对照土壤样品，不能达到土壤微生物所需的养分比例水平。观察到，距井口0~10 m处含油有机碳含量明显高于其他区域，并随着距井距的增加而降低。基于土壤有机碳、全氮、全磷的测定，以及土壤微生物生物量碳（SMBC）、土壤微生物生物量氮（SMBN）和磷含量分析，观察到只有土壤有机碳显着为正与土壤中的油类污染物有关。C 与 N、SMBC 和 SMBN 比率的不平衡会导致比微生物所需的养分严重短缺，限制土壤微生物的繁殖和生长，从而减缓原生微生物降解石油烃的速度，从而降低石油污染对整个生态系统稳定性的影响。因此，在本研究区石油污染土壤修复过程中，需要合理地向土壤中添加足够的养分。