Cell division cycle protein 37 (Cdc37) is a molecular chaperone that actively participates in many intracellular physiological and biochemical processes as well as pathogen infection. However, the function of Cdc37 in silkworm cells under Bombyx mori nucleopolyhedrovirus (BmNPV) infection is unknown. We cloned and identified BmCdc37, a Cdc37 gene from B. mori, which is highly conserved among other species. After BmNPV infection, the expression level of the BmCdc37 gene was up-regulated and showed an expression pattern similar to the BmHsp90 gene, which relies on Cdc37 to stabilize and activate specific protein kinases. The immunofluorescence, bimolecular fluorescence complementation (BiFC), and co-immunoprecipitation (Co-IP) assays all indicated that BmCdc37 interacts with BmHsp90 in silkworm cells. Both BmCdc37 and BmHsp90 promote the reproduction of BmNPV. Co-expression of BmCdc37 and BmHsp90 was better at promoting virus proliferation than overexpression alone. These findings all indicate that BmCdc37 plays an active role in the proliferation of BmNPV.

细胞分裂周期蛋白 37 (Cdc37) 是一种分子伴侣，积极参与许多细胞内生理和生化过程以及病原体感染。然而，Cdc37 在家蚕核多角体病毒 (BmNPV) 感染下的家蚕细胞中的功能尚不清楚。我们克隆并鉴定了BmCdc37，这是一种来自B. mori的Cdc37基因，在其他物种中高度保守。BmNPV感染后，BmCdc37基因的表达水平上调并表现出与BmHsp90相似的表达模式基因，它依赖于 Cdc37 来稳定和激活特定的蛋白激酶。免疫荧光、双分子荧光互补 (BiFC) 和免疫共沉淀 (Co-IP) 检测均表明 BmCdc37 与家蚕细胞中的 BmHsp90 相互作用。BmCdc37 和 BmHsp90 均促进 BmNPV 的繁殖。BmCdc37和BmHsp90 的共表达在促进病毒增殖方面比单独过表达更好。这些发现都表明BmCdc37在BmNPV的增殖中起积极作用。