Background

Thermo-tolerant Campylobacter species are the major cause of foodborne diseases worldwide. This study aimed to evaluate the prevalence of virulence genes and antibiotic resistance determinants in Campylobacter jejuni and Campylobacter coli isolates, and to investigate the relationship between these two traits.

Methods

A total of 132 Campylobacter isolates from poultry were tested for the presence of 13 virulence genes; flaA, cadF, racR, virB11, pldA, dnaJ, cdtA, cdtB, cdtC, ciaB, wlaN, cgtB and ceuE. The mechanisms underlying antibiotic resistance phenotypes were also studied by PCR and MAMA-PCR.

Results

PCR results revealed the presence of antimicrobial resistance genes in C. jejuni and C. coli as follows: cmeB (80% and 100%), tet(O) (100% and 80%), and the bla_OXA-61 (81% and 93%), respectively. None of these strains harbored the aphA-3 gene.

The Thr-86-Ile mutation associated with resistance to quinolones was found in 90% of C. jejuni and 80% of C. coli isolates. While the A2075G and A2074C mutations linked to the erythromycin resistance were detected in 100% of both species.

Virulence genes were prevalent and ranged from 40 to 100%. A positive relationship was revealed between cadF, racR, and ciaB genes and resistance to ampicillin, amoxicillin/clavulanic acid, chloramphenicol, and nalidixic acid, in C. jejuni. However, no association was observed for C. coli isolated strains.

Conclusion

This study provides for the first time an overview of antibiotic resistance mechanisms and pathogenic profiles of Campylobacter isolates, which emphasizes the potential risk for consumer health.

中文翻译：

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突尼斯肉鸡空肠弯曲杆菌和大肠杆菌的毒力和抗生素抗性基因分布

背景

耐热弯曲杆菌是全世界食源性疾病的主要原因。本研究旨在评估空肠弯曲杆菌和大肠弯曲杆菌分离株中毒力基因和抗生素耐药性决定因素的流行程度，并研究这两种性状之间的关系。

方法

总共检测了 132株来自家禽的弯曲杆菌分离株是否存在 13 种毒力基因；flaA、cadF、racR、virB11、pldA、dnaJ、cdtA、cdtB、cdtC、ciaB、wlaN、cgtB和ceuE。还通过 PCR 和 MAMA-PCR 研究了抗生素耐药表型的潜在机制。

结果

PCR 结果显示空肠弯曲杆菌和大肠杆菌中存在抗菌素耐药基因，如下所示：cme B（80% 和 100%）、tet (O)（100% 和 80%）和bla _OXA-61（81 ％和93％），分别。这些菌株均不携带 aph A-3 基因。

在 90% 的空肠弯曲杆菌和 80% 的大肠杆菌分离株中发现了与喹诺酮类耐药相关的 Thr-86-Ile 突变。而与红霉素耐药性相关的 A2075G 和​​ A2074C 突变在两个物种中均被 100% 检测到。

毒力基因普遍存在，范围从 40% 到 100%。在空肠弯曲菌中，cadF、racR和ciaB基因与对氨苄青霉素、阿莫西林/克拉维酸、氯霉素和萘啶酸的耐药性呈正相关。然而，没有观察到大肠杆菌分离菌株的关联。

结论

本研究首次概述了弯曲杆菌分离株的抗生素耐药机制和致病谱，强调了对消费者健康的潜在风险。