Although phosphorus (P) addition and arbuscular mycorrhizal fungal (AMF) colonization have potential for mitigating soil N₂O emission, the effects and mechanisms remain unresolved. We conducted a pot experiment with ryegrass (Lolium perenne) growing in a growth chamber for 80 days with three factors: Plant and AMF presence (unplanted, with plants, with plants colonized by AMF), two nitrogen (N) addition levels (0 and 50 mg N kg⁻¹ soil) and two P addition levels (0 and 20 mg P kg⁻¹ soil). Our results showed that N addition and AMF colonization had insignificant effects on soil N₂O emission. However, the presence of plants decreased soil N₂O emission by 90%–99% (planted vs. unplanted) under P addition. Moreover, P addition increased (+134%) N₂O emission from unplanted soil, but decreased (74%–98%) it from planted soil. Further analysis showed that soil N₂O emission was controlled by soil available P, soil NO₃⁻-N, plant biomass P and N across all treatments. The lower N₂O emissions from planted soils were mainly due to the lower soil NO₃⁻-N content which might be immobilized by plant biomass, while the higher N₂O emissions from unplanted soils under P addition were attributed to the increased soil available P content. Furthermore, we found an antagonistic effect between AMF inoculation and P addition on soil N₂O emission because sufficient soil P may inhibit AMF colonization and activity. Taken together, we conclude that plant presence combined with P addition can effectively reduce N₂O emission from P-limited soils.

尽管磷 (P) 添加和丛枝菌根真菌 (AMF) 定植具有减少土壤 N ₂ O 排放的潜力，但其影响和机制仍未得到解决。我们对在生长室中生长 80 天的黑麦草 ( Lolium perenne )进行了盆栽试验，其中包含三个因素：植物和 AMF 的存在（未种植的、有植物的、植物被 AMF 定殖）、两种氮 (N) 添加水平（0 和50 mg N kg ^-1土壤）和两个 P 添加水平（0 和 20 mg P kg ^-1土壤）。我们的结果表明，N添加和AMF定植对土壤N ₂ O排放的影响不显着。然而，植物的存在降低了土壤 N ₂在 P 添加下，O 排放量增加 90%–99%（种植与未种植）。此外，P添加增加了（+134%）未种植土壤的N ₂ O排放，但减少了（74%–98%）种植土壤的N ₂ O排放。进一步的分析表明，在所有处理中，土壤 N ₂ O 排放受土壤有效磷、土壤 NO ₃ ^- -N、植物生物量 P 和 N 的控制。种植土壤中较低的 N ₂ O 排放主要是由于较低的土壤 NO ₃ ^- -N 含量可能被植物生物量固定，而较高的 N ₂添加磷后未种植土壤的 O 排放归因于土壤有效磷含量的增加。此外，我们发现 AMF 接种和 P 添加之间对土壤 N ₂ O 排放的拮抗作用，因为充足的土壤 P 可能会抑制 AMF 的定植和活性。综上所述，我们得出结论，植物存在与磷添加相结合可以有效减少限磷土壤中的N ₂ O 排放。