Repeatability of hormone concentrations is of great interest for studies investigating the evolution of hormonal traits. Particularly the repeatability of glucocorticoids (GCs) in response to a stressor is frequently investigated, but often only point (initial and/or response value), or single measures are used. A new method takes into account the entire individual hormone profile and generates an individual profile repeatability (PR) score. The method was developed for response profiles, but it may also be valuable for baseline values in species with diurnal changes in hormone concentrations. GCs are determined in a variety of matrices, and repeatability can vary considerably depending on the matrix. We investigated the repeatability of baseline GC metabolite (GCM) concentrations measured in urine (uGCM) and faeces (fGCM) of captive eastern rock sengis (Elephantulus myurus) using the more traditional linear mixed model approach and the PR method. GCMs were assessed over 24 h and measurements were repeated twice with two weeks between replicates. A diurnal rhythm in GCM concentrations associated with the activity period of the sengis was found in urine, but not in the faeces. Urinary GCM concentrations exhibited a moderate repeatability, whereas the repeatability of fGCM concentrations was low. Urinary GCM concentrations and their repeatability differed between the sexes; with higher concentrations and lower PR scores in females. No such sex differences were apparent for fGCM concentrations and the PR score was not able to characterise repeatability of fGCM concentrations, which were lacking a distinct profile. The PR score enabled a successful quantification of the repeatability of the diurnal uGCM profiles. Hormone profile, sex and sample matrix can affect hormonal traits considerably and the results may be obscured if these factors are not carefully considered.

激素浓度的可重复性对于研究激素性状进化的研究非常重要。特别是糖皮质激素 (GC) 对压力源的反应的可重复性经常被研究，但通常只使用点（初始值和/或响应值）或单一测量值。一种新方法考虑了整个个体激素谱并生成个体谱可重复性 (PR) 分数。该方法是为响应曲线开发的，但它也可能对激素浓度昼夜变化的物种的基线值有价值。GC 在多种基质中测定，重复性可能因基质而异。象牙) 使用更传统的线性混合模型方法和 PR 方法。GCM 评估超过 24 小时，测量重复两次，重复间隔两周。在尿液中发现 GCM 浓度的昼夜节律与 sengis 的活动期有关，但在粪便中则没有。尿液 GCM 浓度表现出中等的可重复性，而 fGCM 浓度的可重复性较低。不同性别的尿液 GCM 浓度及其重复性存在差异；在女性中浓度较高且 PR 分数较低。fGCM 浓度没有明显的性别差异，PR 评分无法表征 fGCM 浓度的可重复性，缺乏明显的特征。PR 分数能够成功量化昼夜 uGCM 剖面的可重复性。