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α-l-Arabinofuranosidase as an Orthogonal Enzyme for Human Cells
Chemistry Letters ( IF 1.4 ) Pub Date : 2021-07-17 , DOI: 10.1246/cl.210231
Ryosuke Kaneko, Tsuyoshi Oda, Ryosuke Yoshida, Chuya Tateishi, Kenta Tanito, Teruki Nii, Akihiro Kishimura, Noriho Kamiya, Takeshi Mori, Yoshiki Katayama

Here we proposed α-l-arabinofuranosidase from Clostridium thermocellum as an enzyme for signal amplification in bioanalysis whose activity does not exist in human cells. Combination of this enzyme and β-galactosidase enabled simultaneous detection of two different antigens on live cells.

中文翻译:

α-l-阿拉伯呋喃糖苷酶作为人类细胞的正交酶

在这里,我们提出了来自热纤梭菌的α- l-阿拉伯呋喃糖苷酶作为生物分析中信号放大的酶,其活性在人类细胞中不存在。这种酶和 β-半乳糖苷酶的组合能够同时检测活细胞上的两种不同抗原。
更新日期:2021-07-18
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