Inflammatory response in patients with COPD secondary to organic dust exposure (OD-COPD) is poorly understood. We therefore aimed to characterize inflammatory and immune profile from peripheral blood mononuclear cells (PBMC) in a group of patients with mild-to-moderate COPD secondary to organic dust exposure (OD-COPD), tobacco smoking (T-COPD), or both. We compared T, B and NK cells distribution and inflammatory (TNF-α, Il-1β, IL-6), type 1 (IFN-γ), type 2 (IL-4, IL-13) and type 3 (IL-17) immunity related cytokines at baseline, and after stimulation with LPS, flagellin and CD3/CD28 beads in all COPD groups. OD-COPD displayed significantly lower NK cells and CD8+ T cells compared with controls. After flagellin stimulation, T-COPD had significantly lower IL-13 levels than OD-COPD and controls (p < 0.05) whereas IFN-γ tended to be lower in OD-COPD. All COPD groups displayed higher IL-1β and IL-17 than controls after CD3/CD28 stimulation. Inflammatory responses in OD-COPD were different from T-COPD. OD-COPD displayed higher levels of type 2 immunity related cytokines.

继发于有机粉尘暴露 (OD-COPD) 的 COPD 患者的炎症反应知之甚少。因此，我们旨在表征一组继发于有机粉尘暴露 (OD-COPD)、吸烟 (T-COPD) 或两者兼有的轻度至中度 COPD 患者的外周血单核细胞 (PBMC) 的炎症和免疫特征。 . 我们比较了 T、B 和 NK 细胞分布和炎症（TNF-α、IL-1β、IL-6）、1 型（IFN-γ）、2 型（IL-4、IL-13）和 3 型（IL- 17) 基线时和所有 COPD 组中用 LPS、鞭毛蛋白和 CD3/CD28 珠刺激后的免疫相关细胞因子。与对照组相比，OD-COPD 显示出显着较低的 NK 细胞和 CD8+ T 细胞。鞭毛蛋白刺激后，T-COPD 的 IL-13 水平显着低于 OD-COPD 和对照组（p < 0.05) 而 OD-COPD 中的 IFN-γ 往往较低。在 CD3/CD28 刺激后，所有 COPD 组都显示出比对照组更高的 IL-1β 和 IL-17。OD-COPD 的炎症反应与 T-COPD 不同。OD-COPD 显示出较高水平的 2 型免疫相关细胞因子。