Expression of 20-hydroxyecdysone-related genes during gonadal development of Teleogryllus emma (Orthoptera: Gryllidae),Archives of Insect Biochemistry and Physiology

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Expression of 20-hydroxyecdysone-related genes during gonadal development of Teleogryllus emma (Orthoptera: Gryllidae)
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-07-17 , DOI: 10.1002/arch.21824
Wenjie Jin _{1,

2,

3} , E Tan ₃ , George Ghartey-Kwansah ₃ , Yishu Jia ₃ , Gengsi Xi ₃

Affiliation

Insect gonads develop under endocrine signals. In this study, we assessed the characters of partial complementary DNAs encoding the Teleogryllus emma orthologs of 20-hydroxyecdysone (20E)-related genes (RXR, E75, HR3, Hsc70, and Hsp90) and analyzed their expression patterns in both nymph and adult crickets. 20E treatment suppressed expression of TeEcR, TeRXR, TeE75, TeHR3, TeHsc70, and TeHsp90. Temporal expression analysis demonstrated that TeERR and 20E-related genes were expressed in four stages of gonadal development from the fourth-instar nymph stage to the adult stage. The expression pattern of these genes differed in testicular and ovarian development. TeRXR, HR3, TeHsc70, and TeHsp90 were irregularly expressed in gonads of the same developmental stages, while mRNAs encoding TeERR, TeEcR, and TeE75 accumulated in higher levels in ovaries than in testes. RNA interference (RNAi) of TeEcR expression led to decrease of the expression levels of TeEcR, TeRXR, TeHR3, and TeHsc70, while it enhanced TeE75 and TeHsp90 expressions. These results demonstrate that the TeERR and 20E-related genes help regulate gonadal development, while TeEcR appears to inhibit TeE75 expression, TeE75 inhibits HR3 expression. Hsc70 indirectly regulated the expression of the primary and secondary response genes E74A, E75B, and HR3. Hsp90 regulated Usp expression with no direct regulatory relationship with EcR.

中文翻译：

Teleogryllus emma（直翅目：Gryllidae）性腺发育过程中20-羟基蜕皮激素相关基因的表达

昆虫性腺在内分泌信号下发育。在这项研究中，我们评估了编码20-羟基蜕皮激素(20E) 相关基因（RXR、E75、HR3、Hsc70和Hsp90）的Teleogryllus emma直向同源物的部分互补 DNA 的特征，并分析了它们在若虫和成年蟋蟀中的表达模式. 20E 处理抑制TeEcR、TeRXR、TeE75、TeHR3、TeHsc70和TeHsp90 的表达。时间表达分析表明TeERR和 20E 相关基因在从四龄若虫阶段到成虫阶段的四个性腺发育阶段中表达。这些基因的表达模式在睾丸和卵巢发育中有所不同。TeRXR、HR3、TeHsc70和TeHsp90在相同发育阶段的性腺中不规则表达，而编码TeERR、TeEcR和TeE75 的mRNA在卵巢中的积累水平高于睾丸。TeEcR表达的RNA 干扰 (RNAi)导致TeEcR、TeRXR、TeHR3和TeHsc70的表达水平降低，同时增强TeE75和TeHsp90 的表达。这些结果表明TeERR和 20E 相关基因有助于调节性腺发育，而Te EcR似乎抑制TeE75表达，TeE75 抑制HR3表达。Hsc70 间接调节初级和次级反应基因 E74A、E75B 和 HR3 的表达。Hsp90的调控USP表达没有直接的监管关系的EcR。

更新日期：2021-08-17

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