Single-cell RNA sequencing (scRNA-seq) of the bronchial epithelium enables examination of cellular subtypes and their responses to viral infections. Here, an optimized method for the isolation of virally infected primary bronchial epithelial cells using a commercially available microfluidic device is presented. Using this method single cells can be rapidly isolated with minimal equipment available in most laboratories. Isolation can be carried out inside biological safety cabinets, permitting the use of virally infected cells. Both cell-line and primary cells isolated using the device retained sufficient RNA integrity for the generation of short-read sequencing-compatible cDNA libraries to facilitate scRNA-seq.

中文翻译：

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快速微流体分离病毒感染的原代支气管上皮细胞以进行单细胞 RNA 测序。


支气管上皮的单细胞 RNA 测序 (scRNA-seq) 能够检查细胞亚型及其对病毒感染的反应。在这里，提出了一种使用市售微流体装置分离病毒感染的原代支气管上皮细胞的优化方法。使用这种方法，可以使用大多数实验室可用的最少设备快速分离单细胞。隔离可以在生物安全柜内进行，从而允许使用病毒感染的细胞。使用该设备分离的细胞系和原代细胞都保留了足够的 RNA 完整性，用于生成短读长测序兼容的 cDNA 文库，以促进 scRNA-seq。