To make in vitro single cell electroporation protocols more comparable between various cancer types and groups, we propose a set of assays to test a range of electric field strengths at the start of any new project to determine the optimal electric field strength for a given cell line. While testing a range of electric field strengths, we kept the other ESOPE parameters constant (8 pulses, 100 µs pulse duration, 1 Hz pulse frequency). Basic assays were employed to measure short-term viability, effectiveness of treatment, metabolic activity, and recovery potential post-treatment to determine the optimal field strength for a particular cell line. Six cancer cell lines were tested, three of human (A549, A375 and Pan02) and three murine (LLC, B16F10 and MIA-PACA2). Our findings demonstrate that the optimal electroporation setting while keeping with all other ESOPE parameters are 800 V/cm for A549 and Pan02, 700 V/cm for A375, Mia-PACA2, and B16F10, and 1300 V/cm for LLC. Having an agreed upon set of assays to determine each cell lines optimal electric field strength should allow an improve translation of findings between cell lines for in vitro work from various groups and potentially improve translation into the clinic.

在体外制作单细胞电穿孔方案在各种癌症类型和组之间更具可比性，我们提出了一组测定法，以在任何新项目开始时测试一系列电场强度，以确定给定细胞系的最佳电场强度。在测试一系列电场强度时，我们保持其他 ESOPE 参数不变（8 个脉冲，100 µs 脉冲持续时间，1 Hz 脉冲频率）。使用基本测定来测量短期生存力、治疗效果、代谢活性和治疗后恢复潜力，以确定特定细胞系的最佳场强。测试了六种癌细胞系，三种人（A549、A375 和 Pan02）和三种鼠（LLC、B16F10 和 MIA-PACA2）。我们的研究结果表明，在保持所有其他 ESOPE 参数的同时，最佳电穿孔设置是 A549 和 Pan02 为 800 V/cm，A375、Mia-PACA2 和 B16F10 为 700 V/cm，LLC 为 1300 V/cm。有一套商定的测定方法来确定每个细胞系的最佳电场强度应该允许改进细胞系之间的发现转化为来自各个小组的体外工作，并可能改善临床转化。