Abstract Glutathione-chitosan conjugates are adequate carriers for anticancer treatment due to their ability for inhibition of efflux pumps, improved mucoadhesivity and in-situ gelling. These conjugates were obtained via carbodiimide at different reaction times in order to get different thiolation degrees (i.e., 4.4!, 5.1! and 7.0!) and their behavior in aqueous media at a pH of 4, 5 and 6 was assessed by measurements of hydrodynamic diameter, zeta potential and rheological analyses at a pH ranging from 4 to 6. Data examination was conducted by principal component analysis (PCA) which in turn explained 73.1! of data variability. All samples showed a Newtonian flow and thiolation rendered materials with a highly pronounced temperature-dependent behavior and a gel-like structure. In turn, the phase shift angle was the most prominent rheological change especially at a pH of 5.0 and 6.0 due to the formation of disulfide bonds. The thiolation degree was the most influential factor and it was inversely related to particle charge and consistency index.

中文翻译：

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谷胱甘肽-壳聚糖偶联物在水性介质中的制备、表征和流变行为

摘要 谷胱甘肽-壳聚糖偶联物具有抑制外排泵、改善粘膜粘附性和原位胶凝的能力，是抗癌治疗的合适载体。这些缀合物是通过碳二亚胺在不同反应时间获得的，以获得不同的硫醇化度（即 4.4!、5.1! 和 7.0!），并且它们在 pH 为 4、5 和 6 的水性介质中的行为通过流体力学测量来评估在 4 到 6 的 pH 值范围内进行直径、zeta 电位和流变分析。通过主成分分析 (PCA) 进行数据检查，这反过来解释了 73.1！的数据可变性。所有样品都表现出牛顿流动和硫醇化作用，使材料具有高度显着的温度依赖性行为和凝​​胶状结构。反过来，由于二硫键的形成，相移角是最显着的流变变化，尤其是在 pH 值为 5.0 和 6.0 时。硫醇化度是影响最大的因素，它与粒子电荷和稠度指数呈负相关。