Abstract A better elucidation of molecular mechanisms underlying drug-membrane interaction is of great importance for drug research and development. To date, different biochemical and biophysical methods have been developed to study biological membranes at molecular level. This review focuses on the recent applications and achievements of modern analytical techniques in the study of drug interactions with lipid membranes, including chromatography, spectrometry, calorimetry, and acoustic sensing. The merits and limitations of these techniques were compared and critically discussed. Moreover, various types of biomimetic model membranes including liposomes, lipid monolayers, and supported lipid monolayers/bilayers were described. General mechanisms underlying drug-membrane interaction process were also briefly introduced.

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用于研究药物-脂质膜相互作用的分析技术和方法

摘要 更好地阐明药物-膜相互作用的分子机制对于药物研发具有重要意义。迄今为止，已经开发了不同的生化和生物物理方法来在分子水平上研究生物膜。本综述重点介绍了现代分析技术在研究药物与脂质膜相互作用方面的最新应用和成就，包括色谱法、光谱法、量热法和声学传感。对这些技术的优点和局限性进行了比较和批判性讨论。此外，还描述了各种类型的仿生模型膜，包括脂质体、脂质单层和支持的脂质单层/双层。还简要介绍了药物-膜相互作用过程的一般机制。