Abstract Many of the plant proteins are used as medicinal agents in the treatment of various diseases/disorders as they are produced by using molecular tools of biotechnology. Each protein is unique in its amino acid composition, sequence, subunit structures, size, shape, net charge, iso electric point, solubility, heat stability and hydrophobicity known to play a major role in the isolation and characterization procedures. The study of the protein of interest out of a large number is not possible unless it is obtained in its highly purified and intact form. Extraction, purification and characterization of proteins for different sample types are useful in determining structural, functional and other biological information in the field of pharmacy. Hence, the present review focuses on the sources, isolation, purification and characterization of natural proteins which are proven to be antidiabetic so as to commercialize these drugs (neutraceuticals) to compete with insulin, an ultimate in the treatment of diabetes mellitus.

中文翻译：

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抗糖尿病植物蛋白/肽作为补充和替代药物——分析观点

摘要 许多植物蛋白是通过使用生物技术的分子工具生产的，因此被用作治疗各种疾病/病症的药物。每种蛋白质在其氨基酸组成、序列、亚基结构、大小、形状、净电荷、等电点、溶解性、热稳定性和疏水性方面都是独一无二的，已知这些在分离和表征过程中起主要作用。除非以高度纯化和完整的形式获得，否则不可能对大量感兴趣的蛋白质进行研究。不同样品类型的蛋白质的提取、纯化和表征可用于确定药学领域的结构、功能和其他生物信息。因此，本次审查侧重于来源、隔离、