Demand for therapeutic honey is driving establishment of Leptospermum plantations. This study developed micropropagation methods for two species – Leptospermum polygalifolium Salisb. and L. scoparium J.R.Forst. & G.Forst. The study determined how shoot proliferation and adventitious rooting were influenced by the original explant position on the seedling and the concentration of benzyladenine (BA) in the proliferation medium. Hormone-free node culture was highly effective for both species. Nodal explants often formed roots in the absence of BA and developed elongated axillary shoots. Median shoot numbers of 584 and 659 were formed in 31–32 weeks from a single L. polygalifolium or L. scoparium seed, respectively. A low BA dose was effective for callogenesis and shoot proliferation of L. polygalifolium, but not L. scoparium. The median number of shoots produced from a single L. polygalifolium seed was 630 using 2.22-μM BA. This dose induced extremely high shoot numbers in some clones because explants often produced extensive callus and multiple short shoots. Shoots formed adventitious roots without indole-3-butyric acid and plantlets were acclimatised to nursery conditions. The original explant position did not influence shoot proliferation or adventitious rooting. Leptospermum polygalifolium and L. scoparium proved amenable to micropropagation, facilitating rapid establishment of nectar plantations.

对治疗性蜂蜜的需求推动了Leptospermum种植园的建立。该研究开发了两种物种的微繁殖方法 - Leptospermum polygalifolium Salisb。和L. scoparium JRForst。& G. 福斯特。该研究确定了幼苗上的原始外植体位置和增殖培养基中苄基腺嘌呤 (BA) 的浓度如何影响芽增殖和不定根。无激素节点培养对这两个物种都非常有效。节外植体通常在没有 BA 的情况下形成根并发育出细长的腋芽。584 和 659 的中位数芽数是在 31-32 周内从单一的L. polygalifolium或L. scoparium 形成的种子，分别。低 BA 剂量对L. polygalifolium 的胼胝体形成和枝条增殖有效，但对L. scoparium无效。使用 2.22-μM BA，从单一的远志种子产生的枝条的中位数为 630。该剂量在一些克隆中诱导了极高的枝条数，因为外植体通常产生广泛的愈伤组织和多个短枝。芽在没有 indole-3-butyric 的情况下形成不定根，并且小植株适应了苗圃条件。原始外植体位置不影响芽增殖或不定生根。Leptospermum polygalifolium和L. scoparium 证明适合微繁殖，促进花蜜种植园的快速建立。