The production of biodiesel from oily waste has been reported as a promising alternative for the use of biomass in energy. However, the high content of free fatty acids makes traditional chemical catalysis processes difficult. This work aimed to evaluate the enzymatic production of biodiesel from oily residues derived from sanitation and its optimization for large-scale applications. We evaluated the production of biodiesel from a University Restaurant grease trap using lipases as catalysts (Candida antarctica, Thermomyces lanuginosa, Mucor miehei). The experiments were performed by using a factorial design of type 3² to evaluate the best conditions of temperature (30, 40 and 50 °C) and enzyme concentration (5, 7.5 and 10% w/w) for each different enzyme. When the enzyme obtained from C. antarctica was used, the high yield was obtained in all studied conditions and none of the variables was statistically significant. As for the enzymes from the T. lanuginosa and the M. miehei significant effect of the temperature was reported, the higher yield was obtained with lower temperatures (30–40 °C). The concentration of enzymes only was statistically significant with enzyme from M. miehei, which had better yield with 10% w/w fat. Still, the lowest yields of biodiesel were obtained when using the enzyme from M. miehei. The enzymatic production of biodiesel from oily residues can be an interesting strategy for reducing production costs and an alternative for the sustainable treatment of oily residues from sanitation.

据报道，从含油废物中生产生物柴油是将生物质用于能源的一种有前途的替代方案。然而，游离脂肪酸的高含量使传统的化学催化过程变得困难。这项工作旨在评估从卫生产生的油性残留物中酶促生产生物柴油及其大规模应用的优化。我们评估了使用脂肪酶作为催化剂（南极念珠菌、丝状嗜热丝孢菌、米黑毛霉菌）从大学餐厅隔油池生产生物柴油的情况。通过使用类型 3 ²的因子设计进行实验，以评估每种不同酶的最佳温度条件（30、40 和 50 °C）和酶浓度（5、7.5 和 10% w/w）。当酶从使用 C. antarctica，在所有研究条件下都获得了高产量，并且没有任何变量具有统计学意义。至于来自T. lanuginosa和M. miehei的酶据报道温度有显着影响，在较低温度（30-40°C）下获得更高的产量。只有来自米黑的酶的酶浓度在统计学上显着，其在 10% w/w 脂肪时具有更好的产量。尽管如此，当使用来自M. miehei的酶时，生物柴油的产量最低. 从油性残渣中酶法生产生物柴油可能是降低生产成本的有趣策略，也是可持续处理来自卫生的油性残渣的替代方法。