In this study, a recently proposed NMR standardization approach by ²H integral of deuterated solvent for quantitative multicomponent analysis of complex mixtures is presented. As a proof of principle, the existing NMR routine for the analysis of Aloe vera products was modified. Instead of using absolute integrals of targeted compounds and internal standard (nicotinamide) from ¹H-NMR spectra, quantification was performed based on the ratio of a particular ¹H-NMR compound integral and 2H-NMR signal of deuterated solvent D₂O. Validation characteristics (linearity, repeatability, accuracy) were evaluated and the results showed that the method has the same precision as internal standardization in case of multicomponent screening. Moreover, a dehydration process by freeze drying is not necessary for the new routine. Now, our NMR profiling of A. vera products needs only limited sample preparation and data processing. The new standardization methodology provides an appealing alternative for multicomponent NMR screening. In general, this novel approach, using standardization by 2H integral, benefits from reduced sample preparation steps and uncertainties, and is recommended in different application areas (purity determination, forensics, pharmaceutical analysis, etc.).

在这项研究中，提出了一种最近提出的 NMR 标准化方法，通过氘代溶剂的² H 积分对复杂混合物进行定量多组分分析。作为原理证明，修改了用于分析芦荟产品的现有 NMR 程序。不是使用来自¹ H-NMR 光谱的目标化合物和内标（烟酰胺）的绝对积分，而是基于特定¹ H-NMR 化合物积分与氘化溶剂 D _{2 的}2H-NMR 信号的比率进行量化O. 对验证特性（线性、重复性、准确度）进行了评估，结果表明该方法在多组分筛选的情况下与内标具有相同的精密度。此外，新程序不需要通过冷冻干燥进行脱水过程。现在，我们对A. vera产品的NMR 分析只需要有限的样品制备和数据处理。新的标准化方法为多组分 NMR 筛选提供了一种有吸引力的替代方案。通常，这种使用 2H 积分标准化的新方法受益于减少的样品制备步骤和不确定性，并被推荐用于不同的应用领域（纯度测定、法医学、药物分析等）。