Two-stage anaerobic digestion (TSAD) is a promising strategy for process-specific operation of the distinctive stages of anaerobic digestion to ultimately enhance the process yield and stability. TSAD aids in reducing the inhibitory effects of acidogenesis on methanogenesis and maintains the harmony of the overall process. TSAD of rice wastewater is examined in the present study. Single-stage mono digestion of rice wastewater confronts volatile fatty acid (VFA) accumulation and process instability mainly due to imbalanced C/N ratio, high organic load and biodegradability. This study is an attempt to resolve the glitches by adopting two-stage anaerobic codigestion (TSACoD) with cow dung slurry (CDS) for enhanced process stability, performance and digestate quality. TSACoD reduced the negative interference of VFA production on the performance of methanogens and increased the yield by 58.69% and the total energy recovery by 1.58 times. The optimal ratio of RWW: CDS was observed to be 25:75 (%v/v) and the highest yield of biohydrogen and biomethane was 7.58 mL H₂/g VS and 292 mL CH₄/g VS respectively. This study provides insights on the crucial process conditions to be maintained and monitored for effective anaerobic digestion of highly biodegradable substrates.

两阶段厌氧消化 (TSAD) 是一种有前景的策略，可用于厌氧消化不同阶段的特定工艺操作，以最终提高工艺产量和稳定性。TSAD 有助于降低产酸对产甲烷的抑制作用，并保持整个过程的和谐。本研究检测了水稻废水的 TSAD。水稻废水的单级单级消化面临挥发性脂肪酸 (VFA) 积累和工艺不稳定，主要是由于 C/N 比不平衡、有机负荷高和生物降解性高。本研究试图通过采用牛粪浆 (CDS) 的两阶段厌氧共消化 (TSACoD) 来解决问题，以提高过程稳定性、性能和消化物质量。TSACoD 减少了 VFA 生产对产甲烷菌性能的负面干扰，产量提高了 58.69%，总能量回收率提高了 1.58 倍。观察到 RWW: CDS 的最佳比例为 25:75 (%v/v)，生物氢和生物甲烷的最高产率为 7.58 mL H₂ /g VS 和 292 mL CH ₄ /g VS。这项研究提供了对维持和监测高度生物可降解底物有效厌氧消化的关键工艺条件的见解。