Two enteropathogenic Escherichia coli strains representing novel serotypes and investigation of their roles in adhesion.,Journal of Microbiology and Biotechnology

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Two enteropathogenic Escherichia coli strains representing novel serotypes and investigation of their roles in adhesion.
Journal of Microbiology and Biotechnology ( IF 2.5 ) Pub Date : 2021-07-15 , DOI: 10.4014/jmb.2105.05016
Jing Wang _{1,

2} , HongBo Jiao ₃ , XinFeng Zhang ₄ , YuanQing Zhang ₅ , Na Sun ₆ , Ying Yang ₆ , Yi Wei _{1,

2} , Bin Hu ₆ , Xi Guo _{1,

2}

Affiliation

Enteropathogenic Escherichia coli (EPEC), which belongs to the attaching and effacing diar¬rheagenic E. coli strain, is a major causative agent of life-threatening diarrhoea in infants in devel¬oping countries. Most EPEC isolates correspond to certain O serotypes; however, many strains are non-typeable. Two EPEC strains, EPEC001 and EPEC080, which could not be serotyped during routine detection, were isolated. In this study, we conducted an in-depth characterization of their putative O-antigen gene clusters (O-AGCs), and constructed mutagenesis the O-AGCs to perform functional analysis of O-antigen (OAg) synthesis. Sequence analysis revealed that the occurrence of O-AGCs in EPEC001 and E. coli O132 may be mediated by recombination between them, and EPEC080 and E. coli O2/O50 might acquire each O-AGC from uncommon ancestors. We also indicated that OAg-knockout bacteria were highly adhesive in vitro, except the EPEC001 wzy derivative whose adherent capability was lesser than that of its wild-type strain, providing a direct evidence that OAg plays a key role in EPEC pathogenesis. Together, we identified two EPEC O serotypes in silico and experimentally, and we studied the adherent capabilities of their OAgs, which highlighted the fundamental and pathogenic role of OAg in EPEC.

更新日期：2021-07-17

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