Monocytes are differentiated into macrophages. In this study, mitochondrial DNA copy number (mtDNAcn) levels and downstream events such as the expression of respiratory chain mRNAs were investigated during the phorbol 12-myristate 13-acetate (PMA)-induced differentiation of monocytes. Although PMA treatment increased mtDNAcn, the expression levels of mRNAs encoded in mtDNA were decreased. The levels of mitochondrial transcription factor A mRNA and protein were also decreased. The levels of coenzyme Q10 remained unchanged. These results imply that, although mtDNAcn is considered as a health marker, the levels of mtDNAcn may not always be consistent with the parameters of mitochondrial functions.

单核细胞分化为巨噬细胞。在这项研究中，在佛波醇 12-肉豆蔻酸酯 13-乙酸酯 (PMA) 诱导的单核细胞分化过程中，研究了线粒体 DNA 拷贝数 (mtDNAcn) 水平和下游事件，例如呼吸链 mRNA 的表达。尽管 PMA 处理增加了 mtDNAcn，但 mtDNA 中编码的 mRNA 的表达水平降低。线粒体转录因子 A mRNA 和蛋白质的水平也降低。辅酶 Q10 的水平保持不变。这些结果意味着，尽管 mtDNAcn 被视为健康标志物，但 mtDNAcn 的水平可能并不总是与线粒体功能的参数一致。