Soil toxicity tests are commonly applied using Enchytraeus crypticus to analyze reproductive outputs. However, the traditional method for counting potworms takes a long time due to the significant number of offspring. This paper compares the conventional total counting of E. crypticus juveniles (M1) and an alternative methodology (M2). The proposed methodology (M2) uses a simple random counting method (1/4) for the partial counting of juveniles and total estimation. Chronic bioassays (21 days of exposure) were performed in tropical artificial soil (TAS) using sugarcane vinasse as a hazardous substance. Comparing the final density of juveniles recorded in M1 and M2, no statistical differences were pointed out in either one. Applying analyses based on effective concentration (EC10 and EC50), no statistical differences were identified there either. The t-test showed that there was no statistical difference between the counting methods (M1 and M2) in each treatment (control and dilutions). Moreover, we ran the Tukey test for M1 and M2 methods separately and observed that 100 % of the vinasse showed a statistical difference compared to the control treatment in both (p ≤ 0.05), affirming that independent of the counting method, the ecotoxicological outputs were similar. Therefore, the proposed alternative is a suitable method for bioassay using. E. crypticus in tropical artificial soil, decreasing to 1/4 the total time required for counting.

土壤毒性测试通常使用Enchytraeus crypticus来分析生殖产出。然而，由于后代数量众多，传统的计算potworms的方法需要很长时间。本文比较了E. crypticus的常规总计数青少年（M1）和替代方法（M2）。建议的方法 (M2) 使用简单的随机计数方法 (1/4) 对青少年进行部分计数和总估计。使用甘蔗酒糟作为有害物质，在热带人造土壤 (TAS) 中进行慢性生物测定（暴露 21 天）。比较 M1 和 M2 记录的幼体最终密度，两者均未发现统计学差异。应用基于有效浓度（EC10 和 EC50）的分析，也没有发现统计差异。t 检验表明，每种处理（对照和稀释）中的计数方法（M1 和 M2）之间没有统计学差异。而且，p  ≤ 0.05），确认独立于计数方法，生态毒理学输出是相似的。因此，所提出的替代方案是用于生物测定的合适方法。E. crypticus在热带人造土壤中，减少到计数所需总时间的 1/4。