Aims: In this study experiments were carried out to explore antioxidant, antimicrobial, cytotoxic properties of novel indole derivative 1-ethyl-2-phenyl-3-phenylethyl-3-thiophen-2-yl-1Hindole (EPI) together with its effect on glutathione S-transferases (GST) activities in human liver carcinoma (HepG2) cells.

Background: Indoles probably represent one of the most important heterocyclic structures that have been attracting the interest of many scientists in drug discovery.

Objective: The present study was carried out to explore antioxidant, antimicrobial, cytotoxic properties of novel indole derivative 1-ethyl-2-phenyl-3-phenylethyl-3-thiophen-2-yl-1H-indole (EPI) and its effect on glutathione S-transferases (GST) activities in human liver carcinoma (HepG2) cells.

Materials and Methods: Pd-catalyst Sonogashira coupling reactions, MTT Assay, Antioxidant capacity test, Antimicrobial test, GST enzyme activity test.

Results: 1-ethyl-2-phenyl-3-(phenylethynyl)-1H-indole had antioxidant and antimicrobial properties. It displayed significant induction in glutathione S-transferases (GST) enzyme activity in human liver cancer cell lines (HepG2), but cytotoxic effect on all tested cancer cell lines could not be observed.

Conclusion: All of these results showed that 1-ethyl-2-phenyl-3-(phenylethynyl)-1H-indole had antioxidant and antimicrobial properties without cytotoxic effect, which could make it a promising active component with further studies.

目的：本研究通过实验研究新型吲哚衍生物 1-乙基-2-苯基-3-苯基乙基-3-噻吩-2-基-1吲哚（EPI）的抗氧化、抗菌、细胞毒特性及其对人体的影响。人肝癌 (HepG2) 细胞中的谷胱甘肽 S-转移酶 (GST) 活性。

背景：吲哚可能是最重要的杂环结构之一，吸引了许多科学家对药物发现的兴趣。

目的：本研究旨在探讨新型吲哚衍生物 1-乙基-2-苯基-3-苯乙基-3-噻吩-2-基-1H-吲哚 (EPI) 的抗氧化、抗菌和细胞毒特性及其对人体的影响。人肝癌 (HepG2) 细胞中的谷胱甘肽 S-转移酶 (GST) 活性。

材料与方法：Pd-催化剂Sonogashira偶联反应、MTT分析、抗氧化能力测试、抗菌测试、GST酶活性测试。

结果：1-乙基-2-苯基-3-(苯基乙炔基)-1H-吲哚具有抗氧化和抗菌特性。它在人肝癌细胞系 (HepG2) 中显示出对谷胱甘肽 S-转移酶 (GST) 酶活性的显着诱导，但未观察到对所有测试癌细胞系的细胞毒性作用。

结论：所有这些结果表明，1-乙基-2-苯基-3-(苯乙炔基)-1H-吲哚具有抗氧化和抗菌特性，且无细胞毒作用，可使其成为有前景的活性成分，有待进一步研究。