Improvement of Conversion Efficiency from d-Glucose to d-Allulose by Whole-Cell Catalysts with Deep Eutectic Solvents,ACS Food Science & Technology

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Improvement of Conversion Efficiency from d-Glucose to d-Allulose by Whole-Cell Catalysts with Deep Eutectic Solvents
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2021-07-16 , DOI: 10.1021/acsfoodscitech.1c00194
Xiaoyan Chen ₁ , Fan Wang _{1,

2} , Shijie Liu ₃ , Qiang Yu ₄ , Wen Wang ₁ , Yu Zhang ₁ , Zhongming Wang ₁ , Zhenhong Yuan ₁

Affiliation

The synthesis efficiency of d-allulose, an ideal sugar substitute and dietary supplement, from d-glucose with whole-cell catalysts was improved by three out of the four tested deep eutectic solvents (DESs) based on choline chloride (ChCl). The promotion effects on the product yields with increments of up to 2.6-fold were observed in reactions with the presence of DESs in the order ChCl/glycerol < ChCl/urea < ChCl/ethylene glycol. DES compositions displayed distinct influences, but all positive effects appeared in the reactions with low DES contents (1–10%). Higher DES contents produced negative effects, and 40% even totally suppressed the reaction. The enhancement of efflux of intracellular molecules was proved by an analysis of the nucleic acid and protein contents in the medium. DES assisted in the increase in cell membrane permeability rather than in the damage of cell wall integrity, accelerating quick contacts between the enzyme and substrate and improving the conversion rate.

中文翻译：

使用深共晶溶剂的全细胞催化剂提高从 d-葡萄糖到 d-阿洛酮糖的转化效率

d-阿洛酮糖的合成效率，一种理想的糖替代品和膳食补充剂，来自d四种测试的基于氯化胆碱 (ChCl) 的低共熔溶剂 (DES) 中的三种改善了全细胞催化剂的葡萄糖。在存在 DES 的反应中，按照 ChCl/甘油 < ChCl/尿素 < ChCl/乙二醇的顺序观察到对产物产率的促进作用，增量高达 2.6 倍。DES 组成显示出明显的影响，但所有积极影响都出现在低 DES 含量（1-10%）的反应中。较高的 DES 含量会产生负面影响，40% 甚至完全抑制了反应。通过对培养基中核酸和蛋白质含量的分析证明了细胞内分子外排的增强。DES有助于增加细胞膜通透性而不是破坏细胞壁完整性，

更新日期：2021-08-20

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