Pharmaceutical active compounds (PhACs) belonging to analgesics, antibiotics, and non-steroidal anti-inflammatory drugs (NSAIDs) therapeutic classes were monitored in wastewater influents and effluents from two Portuguese urban wastewater treatment plants (UWWTPs) for 24 months. Both facilities were chosen due to their effluents are discharged in highly touristic and sensitive areas, Tagus river and Ria Formosa coastal lagoon, respectively. Target PhACs, acetaminophen, diclofenac, ibuprofen, naproxen, sulfadiazine, and sulfamethoxazole were measured using solid-phase extraction (SPE) coupled to liquid chromatography tandem mass spectrometry (LC-MS/MS). PhACs occurrence in the influents was higher than 98%, with acetaminophen presenting the highest concentrations, with values between 16.3 µg/L and 124 µg/L. In the effluents, distinct behavior was observed with diclofenac and sulfamethoxazole showing recalcitrant characteristics, whereas acetaminophen, ibuprofen, and naproxen showed removal efficiencies above 95%. Acetaminophen and ibuprofen amount in influents showed consistently higher concentration levels in autumn (in Beirolas and Faro Nw UWWTPs) and winter (only in Beirolas UWWTP) seasons. These seasonal trends were observed to a greater extent in Beirolas UWWTP than in Faro Nw UWWTP. This study enabled the comprehensive definition of a behavior pattern for these target contaminants, contributing to better characterization and build-up a library of PhACs occurrence. It also allowed a robust seasonal profiling of the target compounds due to the high number of samples analyzed by each season and a longer monitoring campaign, making the obtained results more significant.

在来自两个葡萄牙城市污水处理厂 (UWWTP) 的污水进水和流出物中，对属于镇痛药、抗生素和非甾体抗炎药 (NSAID) 治疗类别的药物活性化合物 (PhAC) 进行了 24 个月的监测。之所以选择这两个设施，是因为它们的污水分别排放到旅游胜地和敏感地区，塔霍河和 Ria Formosa 沿海泻湖。目标 PhAC、对乙酰氨基酚、双氯芬酸、布洛芬、萘普生、磺胺嘧啶和磺胺甲恶唑使用固相萃取 (SPE) 耦合液相色谱串联质谱 (LC-MS/MS) 进行测量。进水中的 PhACs 发生率高于 98%，对乙酰氨基酚浓度最高，介于 16.3 µg/L 和 124 µg/L 之间。在污水中，观察到双氯芬酸和磺胺甲恶唑表现出顽固特征的不同行为，而对乙酰氨基酚、布洛芬和萘普生的去除效率超过 95%。在秋季（Beirolas 和 Faro Nw UWWTPs）和冬季（仅在 Beirolas UWWTPs）季节，流入物中的对乙酰氨基酚和布洛芬含量始终较高。这些季节性趋势在贝罗拉斯 UWWTP 比在 Faro Nw UWWTP 更明显。这项研究能够全面定义这些目标污染物的行为模式，有助于更好地表征和建立 PhACs 发生库。由于每个季节分析的样本数量多且监测活动较长，因此它还允许对目标化合物进行稳健的季节性分析，