Laryngeal squamous cell carcinoma (LSCC) is an aggressive type of head and neck squamous cell carcinoma (HNSCC) with a relatively high rate of morbidity and mortality. An altered miR-144-3p level in LSCC with a small number of patients has been previously reported. However, the clinical implication of miR-144-3p and its involved mechanism underlying this disease is not clearly elucidated. In this work, we aimed to confirm the expression of miR-144-3p with larger samples and also to identify target genes for the investigation of the underlying mechanism of miR-144-3p in LSCC. The levels of miR-144-3p were downregulated in 155 samples of LSCC tissues as compared to 26 non-LSCC samples (SMD: -0.78; 95% confidence interval (CI): -1.23, -0.32). The AUC of 0.90 in the summarized ROC curve also indicated a potential ability to differentiate LSCC from non-LSCC tissues, with a sensitivity of 0.78 and a specificity of 0.88. With respect to the molecular mechanism, we predicted the potential targets from online-based prediction, peer-reviewed publications, and RNA-seq and microarray data. In particular, the genes influenced by transfection with miR-144-3p in the LSCC FaDu cell line were collected from the microarray GSE56243. Lastly, 12 novel targets for miR-144-3p in LSCC were obtained by different algorithms. In conclusion, our study confirmed the loss or downregulation of miR-144-3p in LSCC, which might contribute to the LSCC tumorigenesis and progression via regulation of the 12 novel targets, such as IL24, ITGA6, and CEP55. In the future, further investigations are required to validate the present results.

中文翻译：

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喉鳞状细胞癌中miR-144-3p表达及其靶点的综合评价

喉鳞状细胞癌 (LSCC) 是一种侵袭性的头颈部鳞状细胞癌 (HNSCC)，发病率和死亡率相对较高。先前已经报道了 LSCC 中 miR-144-3p 水平在少数患者中发生改变。然而，miR-144-3p 的临床意义及其参与该疾病的机制尚不清楚。在这项工作中，我们旨在用更大的样本确认 miR-144-3p 的表达，并确定靶基因以研究 miR-144-3p 在 LSCC 中的潜在机制。与 26 个非 LSCC 样本相比，155 个 LSCC 组织样本中 miR-144-3p 的水平下调（SMD：-0.78；95% 置信区间 (CI)：-1.23，-0.32）。AUC 为 0。汇总的 ROC 曲线中的 90 还表明具有区分 LSCC 与非 LSCC 组织的潜在能力，灵敏度为 0.78，特异性为 0.88。关于分子机制，我们从基于在线的预测、同行评审的出版物以及 RNA-seq 和微阵列数据中预测了潜在的目标。特别是，在 LSCC FaDu 细胞系中受 miR-144-3p 转染影响的基因是从微阵列 GSE56243 中收集的。最后，通过不同算法获得了 LSCC 中 miR-144-3p 的 12 个新靶点。总之，我们的研究证实了 LSCC 中 miR-144-3p 的丢失或下调，这可能通过调节 12 个新靶点（例如 IL24、ITGA6 和 CEP55）促进 LSCC 肿瘤的发生和进展。在将来，