Nucleoside diphosphate kinases (NDPKs) are widespread nucleotide-metabolizing enzymes that are involved in a variety of biological processes, including responses to oxidative stress. Although studies have been conducted on NDPKs in mammals and some plants, there is scant research on insect NDPKs, especially in honey bees. In the present study, we isolated AccNDPK from Apis cerana cerana. Sequence analysis showed that AccNDPK has high homology with many NDPKs and contains a highly conserved NDPK active site motif. Based on phylogenetic analysis, AccNDPK has a relatively recent evolutionary relationship with NDPKs in other hymenopteran insects. AccNDPK was found to be highly expressed in newly emerged honey bees and muscle tissues, and RT-qPCR analysis and bacteriostatic assays showed that the expression level of AccNDPK is affected by abnormal temperature, UV light, H₂O₂, heavy metals, and various pesticides. After AccNDPK knockdown, antioxidant-related genes, including AccCAT, AccCYP4G11, AccGSTS4, AccTpx1 and AccMsrA, were upregulated, whereas AccGSTD, AccGST1, AccHSP22.6 and AccTrx1 were downregulated. Furthermore, catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) activities were significantly increased, and the tolerance of bees to oxidative stress caused by cyhalothrin was reduced by silencing of AccNDPK. Given these findings, we speculate that AccNDPK plays an important role in the oxidative stress response of A. cerana cerana.

核苷二磷酸激酶 (NDPKs) 是广泛的核苷酸代谢酶，参与各种生物过程，包括对氧化应激的反应。尽管已经对哺乳动物和一些植物中的 NDPKs 进行了研究，但对昆虫 NDPKs 的研究很少，尤其是在蜜蜂中。在本研究中，我们从中华蜜蜂中分离出了 AccNDPK 。序列分析表明，AccNDPK 与许多 NDPK 具有高度同源性，并含有高度保守的 NDPK 活性位点基序。根据系统发育分析，AccNDPK 与其他膜翅目昆虫中的 NDPKs 具有相对较新的进化关系。加速器NDPK发现在新出现的蜜蜂和肌肉组织中高表达，RT-qPCR分析和抑菌试验表明AccNDPK的表达水平受异常温度、紫外线、H ₂ O ₂、重金属和各种农药的影响. 之后AccNDPK击倒，抗氧化有关的基因，包括AccCAT，AccCYP4G11，AccGSTS4，AccTpx1和AccMsrA，被上调，而AccGSTD，AccGST1，AccHSP22 0.6和AccTrx1被下调。此外，过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD) 和过氧化物酶 (POD) 活性显着增加，蜜蜂对三氟氯氰菊酯引起的氧化应激的耐受性因AccNDPK的沉默而降低。鉴于这些发现，我们推测 AccNDPK 在A. cerana cerana的氧化应激反应中起重要作用。