The suspended sludge and carrier-attached biofilms of three different hybrid moving bed biofilm reactor (MBBR) systems were investigated with respect to their transformation potential for a broad range of micropollutants (MPs) as well as their microbial community composition. For this purpose, laboratory-scale batch experiments were conducted with the separated suspended sludge and the carrier-attached biofilm of every system in triplicate. For all batches the removal of 31 MPs as well as the composition of the microbial community were analyzed. The carrier-attached biofilms from two hybrid MBBR systems showed a significant higher overall transformation potential in comparison to the respective suspended sludge. Especially for the MPs trimethoprim, diclofenac, mecoprop, climbazole and the human metabolite 10,11-dihydro-10-hydroxycarbamazepine consistently higher pseudo-first-order transformation rates could be observed in all three systems. The analysis of the taxonomic composition revealed taxa showing higher relative abundances in the carrier-attached biofilms (e. g. Nitrospirae and Chloroflexi) and in the suspended biomasses (e. g. Bacteroidetes and Betaproteobacteria). Correlations of the biodiversity indices and the MP biotransformation rates resulted in significant positive associations for 11 compounds in suspended sludge, but mostly negative associations for the carrier-attached biofilms. The distinct differences in MP removal between suspended sludge and carrier-attached biofilm of the three different MBBR systems were also reflected by a statistically significant link between the occurrence of specific bacterial taxa (Acidibacter, Nitrospira and Rhizomicrobium) and MP transformation rates of certain MPs. Even though the identified correlations might not necessarily be of causal nature, some of the identified taxa might serve as suitable indicators for the transformation potential of suspended sludge or carrier-attached biofilms.

研究了三种不同混合移动床生物膜反应器 (MBBR) 系统的悬浮污泥和载体附着生物膜，研究了它们对多种微污染物 (MP) 的转化潜力及其微生物群落组成。为此，实验室规模的批量实验对分离的悬浮污泥和每个系统的载体附着生物膜进行了三次重复。对于所有批次，分析了 31 个 MP 的去除以及微生物群落的组成。与各自的悬浮污泥相比，来自两个混合 MBBR 系统的载体附着生物膜显示出显着更高的整体转化潜力。特别是对于 MPs 甲氧苄氨嘧啶、双氯芬酸、甲氯丙酸、克洛姆唑和人体代谢物 10，在所有三个系统中都可以观察到 11-二氢-10-羟基卡马西平始终较高的伪一级转化率。分类学组成的分析显示，在附着于载体的生物膜（例如 Nitrospirae 和 Chloroflexi）和悬浮生物质（例如 Bacteroidetes 和 Betaproteobacteria）中，分类群显示出较高的相对丰度。生物多样性指数和 MP 生物转化率的相关性导致悬浮污泥中 11 种化合物的显着正相关，但主要是载体附着生物膜的负相关。三种不同 MBBR 系统的悬浮污泥和载体附着生物膜之间 MP 去除的明显差异也反映在特定细菌分类群的发生之间的统计显着联系上。酸杆菌、硝化螺菌和根际微生物）和某些 MP 的 MP 转化率。尽管确定的相关性可能不一定具有因果关系，但一些确定的分类群可能作为悬浮污泥或载体附着生物膜转化潜力的合适指标。