Individual identification of horses for pedigree verification and registration is important for the sustainable development of the horse industry. Horse individual identification and parentage tests commonly use the 17 short tandem repeats (STRs) recommended by the International Society for Animal Genetics (ISAG) and the locus LEX33. While many multiplex STR typing systems have been established for the horse, a sex determining marker is usually absent, and none of them can simultaneously detect all 17 ISAG recommended loci and the locus LEX33. Here, we present a 19-plex STR typing system that contains the 17 ISAG recommend loci, the locus LEX33 and amelogenin as sex determining loci. The results of our sensitivity, species specificity, stutter analysis and population data analysis, indicate that this system is a specific, sensitive, and robust tool for the identification of individuals, parentage testing and genetic research in the horse.

中文翻译：

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开发用于马 (Equus caballus) 个体识别和亲子鉴定的 19 重短串联重复分型系统

对马匹进行个体鉴定以进行谱系验证和登记对于马业的可持续发展具有重要意义。马个体识别和亲子鉴定通常使用国际动物遗传学会 (ISAG) 推荐的 17 个短串联重复序列 (STR) 和基因座 LEX33。虽然已经为马建立了许多多重 STR 分型系统，但通常不存在性别决定标记，并且它们都不能同时检测所有 17 个 ISAG 推荐的基因座和基因座 LEX33。在这里，我们提出了一个 19 重 STR 分型系统，其中包含 17 个 ISAG 推荐位点、位点 LEX33 和牙釉蛋白作为性别决定位点。我们的敏感性、物种特异性、口吃分析和种群数据分析的结果表明，该系统是一个特定的、敏感的、