This short review considers to what extent posttranscriptional steps of gene expression can provide the basis for novel control mechanisms and procedures in synthetic biology and biotechnology. The term biocircuitry is used here to refer to functionally connected components comprising DNA, RNA or proteins. The review begins with an overview of the diversity of devices being developed and then considers the challenges presented by trying to engineer more scaled-up systems. While the engineering of RNA-based and protein-based circuitry poses new challenges, the resulting ‘toolsets’ of components and novel mechanisms of operation will open up multiple new opportunities for synthetic biology. However, agreed procedures for standardization will need to be placed at the heart of this expanding field if the full potential benefits are to be realized.

中文翻译：

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酿酒酵母转录后生物电路的工程化和标准化

这篇简短的综述考虑了基因表达的转录后步骤在多大程度上可以为合成生物学和生物技术中的新控制机制和程序提供基础。此处使用的术语生物电路是指功能连接的组件，包括 DNA、RNA 或蛋白质。审查首先概述了正在开发的设备的多样性，然后考虑了尝试设计更多放大系统所带来的挑战。虽然基于 RNA 和基于蛋白质的电路工程提出了新的挑战，但由此产生的组件“工具集”和新的操作机制将为合成生物学开辟多个新机遇。然而，如果要实现全部潜在利益，就需要将商定的标准化程序置于这个不断扩大的领域的核心。