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Diversity and Pathogenicity of Pectobacterium Species Responsible for Causing Soft Rot and Blackleg of Potato in the Columbia Basin
American Journal of Potato Research ( IF 1.5 ) Pub Date : 2021-07-14 , DOI: 10.1007/s12230-021-09841-4
Hannah M. Rivedal 1 , Jessie A. Brazil 2 , Kenneth E. Frost 2, 3
Affiliation  

Pectobacterium and Dickeya species cause soft rot and blackleg diseases of potato which result in yield losses throughout the United States. The bacterial species involved in this disease complex have not been recently determined in the Columbia Basin. In 2018 and 2019, 145 plant samples with symptoms of soft rot were analyzed for the bacterial species causing disease. Soft rot bacteria species were detected in 51 samples using PCR, including P. carotovorum (74.1%), P. atrosepticum (20.4%), and Dickeya species (5.6%). However, additional phylogenetic assessment of three gene sequences detected several isolates of P. parmentieri and P. brasiliense, originally identified as P. carotovorum. Pathogenicity was assessed by inoculation of tubers and stems. P. parmentieri and P. carotovorum caused high incidence of tuber rot and severity which was enhanced when co-inoculated with other species. P. atrosepticum caused high incidence of stem rot. Pectobacterium spp. were the predominant soft rot pathogens of Columbia Basin potato, and their diversity has implications for accurate diagnostics.



中文翻译:

导致哥伦比亚盆地马铃薯软腐病和黑腿病的果杆菌属物种的多样性和致病性

PectobacteriumDickeya物种会导致马铃薯软腐病和黑腿病,从而导致整个美国的产量损失。最近在哥伦比亚盆地尚未确定参与这种疾病复合体的细菌种类。2018 年和 2019 年,对 145 份有软腐病症状的植物样本进行了致病菌种分析。在使用PCR 51克的样品,包括进行检测软腐病菌物种P. carotovorum(74.1%),P。atrosepticum(20.4%) 的Dickeya物种(5.6%)。然而,对三个基因序列的额外系统发育评估检测到了几株 P. parmentieriP. brasiliense,最初确定为P. carotovorum。通过接种块茎和茎来评估致病性。P. parmentieriP. carotovorum引起块茎腐烂的高发生率和严重程度,当与其他物种共同接种时,这种情况会增强。P. atrosepticum引起茎腐病的高发。果胶杆菌属 是哥伦比亚盆地马铃薯的主要软腐病病原体,它们的多样性对准确诊断有影响。

更新日期:2021-07-15
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