Purpose

Most eukaryotic cells have a plasma membrane with glycoproteins on the outer leaflet and cytoplasmic proteins on the inner leaflet. However, the microsporidians examined have a peculiar outer membrane with cytoplasmic proteins on the surface. His study was to determine if this is true and identify the presence of key cytoplasmic proteins on the exterior of the sporoplasm and spore stages.

Methods

Specific probes including GFP-labeled genes, antibodies, and electron microscopy were applied to studies on the sporoplasm and spore stages of different microsporidian species.

Results

Cytoplasmic membrane proteins cadherin, intermediate filaments, dynactin p150Glued and tubulin were identified on the outer leaflet of the exterior membrane of the sporoplasm or spore stages of Spraguea americanus, Anncaliia algerae, Ameson michaelis and Thelohania sp.

Conclusion

The invasive sporoplasm stage of microsporidians acquires an outer membrane from a cytoplasmic organelle and it has been shown that the outside surface of this membrane bears cytoplasmic proteins. This membrane retains these cytoplasmic proteins even on the spore stage. For an intracellular parasite that locates directly within the cytoplasm of a host cell, the presence of these cytoplasmic proteins on the surface of the parasite appears to have a significant effect on the host’s response.

中文翻译：

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微孢子虫有一个带有外部细胞质蛋白的特殊外膜

目的

大多数真核细胞有一个质膜，外叶上有糖蛋白，内叶上有细胞质蛋白。然而，所检查的微孢子虫具有特殊的外膜，其表面具有细胞质蛋白。他的研究是确定这是否属实，并确定孢子质和孢子阶段外部是否存在关键的细胞质蛋白。

方法

包括 GFP 标记的基因、抗体和电子显微镜在内的特定探针被应用于不同微孢子虫物种的孢子质和孢子阶段的研究。

结果

胞质膜蛋白钙粘蛋白、中间丝、dynactin p150Glued 和微管蛋白在美洲大蠊、Anncaliia algerae、Ameson michaelis和Thelohania sp.的孢子质或孢子阶段的外膜外叶上被鉴定。

结论

微孢子虫的侵入性孢子质阶段从细胞质细胞器获得外膜，并且已经表明该膜的外表面带有细胞质蛋白。即使在孢子阶段，该膜也保留了这些细胞质蛋白。对于直接位于宿主细胞细胞质内的细胞内寄生虫，这些细胞质蛋白在寄生虫表面的存在似乎对宿主的反应有显着影响。