The transcription-related DNA damage response was visualized on a genome-wide scale with great spatial and temporal resolution. Upon UV irradiation, a small proportion of mature RNA transcripts undergo changes, with significant activation of DNA repair factors. Notably, an increase of chromatin accessibility is observed at the immediate early recovery phase and serves as binding sites for selective stage-specific transcription factors. Whole genome analysis of DNA methylation (5mC) delineates pervasive dynamics during DNA repair process, and hypomethylation at gene bodies and 3’UTR is accompanied by induction of DNA damage response genes. Furthermore, temporal-specific m⁶A RNA methylation has been defined and appears to affect DNA repair by modulation of translation. These findings provide a resource for identifying players required for transcription-coupled nucleotide excision repair and reveal insights into the epigenetic regulation of the transcriptional programs in response to genotoxic stress.

转录相关的 DNA 损伤反应在全基因组范围内可视化，具有很高的空间和时间分辨率。在紫外线照射下，一小部分成熟的 RNA 转录物发生变化，DNA 修复因子显着激活。值得注意的是，在立即早期恢复阶段观察到染色质可及性的增加，并作为选择性阶段特异性转录因子的结合位点。DNA 甲基化 (5mC) 的全基因组分析描绘了 DNA 修复过程中普遍存在的动态，基因体和 3'UTR 的低甲基化伴随着 DNA 损伤反应基因的诱导。此外，时间特定的 m ⁶RNA 甲基化已被定义并且似乎通过调节翻译影响 DNA 修复。这些发现为识别转录偶联核苷酸切除修复所需的参与者提供了资源，并揭示了对响应基因毒性压力的转录程序的表观遗传调控的见解。