Probiotics and Antimicrobial Proteins ( IF 4.4 ) Pub Date : 2021-07-15 , DOI: 10.1007/s12602-021-09818-z Kyoung Jun Han 1 , Na-Kyoung Lee 1 , Hyung-Seok Yu 1 , Hoon Park 2 , Hyun-Dong Paik 1
In this study, we investigated the probiotic properties and anti-obesity effects of bacterial strains isolated from homemade kimchi. Lactiplantibacillus plantarum KU15117 was isolated using lactobacilli selective medium. L. plantarum KU15117 did not produce β-glucuronidase and showed high tolerance to artificial gastric juice and bile salt, acceptable resistance to antibiotics, and high adhesion ability to HT-29 cells. The anti-adipogenic activity of L. plantarum KU15117 at 109 CFU/well was confirmed by the reduction of oil red O staining and intracellular triglyceride level. Additionally, the expression levels of fatty acid synthase, CCAAT/enhance-binding protein-α, and peroxisome proliferator-activated receptor-γ, which are associated with the early stage of adipocyte differentiation, were significantly lower in the probiotic-treated group than in the control group. These results suggest that L. plantarum KU15117 has probiotic properties and anti-obesity effects and could be used as a prophylactic probiotics.
中文翻译:
益生菌植物乳杆菌 KU15117 对 3T3-L1 脂肪细胞的抗脂肪生成作用
在这项研究中,我们研究了从自制泡菜中分离出的菌株的益生菌特性和抗肥胖作用。使用乳杆菌选择性培养基分离植物乳杆菌KU15117。植物乳杆菌KU15117 不产生 β-葡萄糖醛酸酶,对人工胃液和胆汁盐具有高耐受性,对抗生素具有可接受的抗性,对 HT-29 细胞具有高粘附能力。植物乳杆菌KU15117 在 10 9时的抗脂肪生成活性 通过油红 O 染色和细胞内甘油三酯水平的降低证实了 CFU/孔。此外,与脂肪细胞分化早期相关的脂肪酸合酶、CCAAT/增强结合蛋白-α和过氧化物酶体增殖物激活受体-γ的表达水平在益生菌处理组中显着低于对照组。对照组。这些结果表明,植物乳杆菌KU15117 具有益生菌特性和抗肥胖作用,可用作预防性益生菌。