Rosemary (Rosmarinus officinalis) is a culinary and medicinal plant used in food and pharmaceutical industry. The wide range of biological activities is mainly related to phenolic and terpenic compounds; like carnosic acid (CA), carnosol (CS) and rosmarinic acid (RA), mainly reported in rosemary leaf extracts, and recently described in rosemary callus extracts. The aim of this work was to investigate the chemical profile of rosemary cell lines and evaluate their antiproliferative potential against human HT-29 colorectal cancer cell lines. For this purpose, rosemary leaf explants were dedifferentiated on MS medium and added with 2, 4-D (2, 4-dichlorophenoxyacetic acid; 2 mg/L) and BAP (6-benzylaminopurine; 2 mg/L). Cell aggregates were separated according to colour and three rosemary cell lines cultures were established: green (RoG), yellow (RoY) and white (RoW). The chemical profile of rosemary cell lines extracts was characterized by combining HPLC and GC platforms coupled to HR-MS/MS. The antiproliferative activity against HT-29 cell line was analyzed with MTT assay. A total of 71 compounds, including hydroxycinnamic acid and hydroxybenzoic acid derivatives, flavonoids, phenolic di- and triterpenes, as well as relevant unsaturated fatty acids and their esters, phytosterols, and carotenoids were tentatively identified in the extract of the target cell lines. The antiproliferative activity test against HT-29 cell using the MTT assay revealed that the viability of HT-29 colon cancer cells was affected after treatment with the RoW extract (IC50 of 49.63 μg/mL) at 48 h. These results showed that rosemary cell lines can also accumulate other bioactive phytochemicals with demonstrated antiproliferative potential.

迷迭香（Rosmarinus officinalis)是一种用于食品和制药工业的烹饪和药用植物。广泛的生物活性主要与酚类和萜类化合物有关；如鼠尾草酸 (CA)、鼠尾草酚 (CS) 和迷迭香酸 (RA)，主要在迷迭香叶提取物中报道，最近在迷迭香愈伤组织提取物中有所描述。这项工作的目的是研究迷迭香细胞系的化学特征并评估它们对人 HT-29 结肠直肠癌细胞系的抗增殖潜力。为此，迷迭香叶外植体在 MS 培养基上去分化，并添加 2, 4-D（2, 4-二氯苯氧乙酸；2 mg/L）和 BAP（6-苄氨基嘌呤；2 mg/L）。根据颜色分离细胞聚集体并建立三种迷迭香细胞系培养物：绿色（Ro G）、黄色（Ro Y) 和白色 ( RoW)。迷迭香细胞系提取物的化学特征通过结合 HPLC 和 GC 平台与 HR-MS/MS 进行表征。用MTT法分析对HT-29细胞系的抗增殖活性。在靶细胞系的提取物中初步鉴定出 71 种化合物，包括羟基肉桂酸和羟基苯甲酸衍生物、黄酮类化合物、酚类二萜和三萜，以及相关的不饱和脂肪酸及其酯、植物甾醇和类胡萝卜素。使用 MTT 测定对 HT-29 细胞的抗增殖活性测试显示，在用 RoW 提取物（IC50 为 49.63 μg/mL）处理 48 小时后，HT-29 结肠癌细胞的活力受到影响。