Methods for enzyme library creation: Which one will you choose?,BioEssays

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Methods for enzyme library creation: Which one will you choose?
BioEssays ( IF 3.2 ) Pub Date : 2021-07-15 , DOI: 10.1002/bies.202100052
Lorea Alejaldre _{1,

2} , Joelle N Pelletier _{1,

2,

3} , Daniela Quaglia _{3,

4}

Affiliation

Enzyme engineering allows to explore sequence diversity in search for new properties. The scientific literature is populated with methods to create enzyme libraries for engineering purposes, however, choosing a suitable method for the creation of mutant libraries can be daunting, in particular for the novices. Here, we address both novices and experts: how can one enter the arena of enzyme library design and what guidelines can advanced users apply to select strategies best suited to their purpose? Section I is dedicated to the novices and presents an overview of established and standard methods for library creation, as well as available commercial solutions. The expert will discover an up-to-date tool to freshen up their repertoire (Section I) and learn of the newest methods that are likely to become a mainstay (Section II). We focus primarily on in vitro methods, presenting the advantages of each method. Our ultimate aim is to offer a selection of methods/strategies that we believe to be most useful to the enzyme engineer, whether a first-timer or a seasoned user.

中文翻译：

酶库创建方法：您会选择哪一种？

酶工程允许探索序列多样性以寻找新的特性。科学文献中充斥着为工程目的创建酶文库的方法，但是，选择合适的方法来创建突变文库可能会令人生畏，尤其是对于新手而言。在这里，我们面向新手和专家：如何进入酶库设计领域，高级用户可以应用哪些指南来选择最适合其目的的策略？第 I 部分专门针对新手，并概述了用于库创建的已建立和标准方法，以及可用的商业解决方案。专家将发现一个最新的工具来更新他们的曲目（第 I 部分）并了解可能成为主流的最新方法（第 II 部分）。我们主要关注体外方法，展示每种方法的优势。我们的最终目标是提供一系列我们认为对酶工程师最有用的方法/策略，无论是初学者还是经验丰富的用户。

更新日期：2021-07-23

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