Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin,Advanced Healthcare Materials

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Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2021-07-15 , DOI: 10.1002/adhm.202100506
Julian C Brandmeier ₁ , Kirsti Raiko ₂ , Zdeněk Farka _{1,

3} , Riikka Peltomaa _{1,

2} , Matthias J Mickert ₁ , Antonín Hlaváček ₄ , Petr Skládal ₃ , Tero Soukka ₂ , Hans H Gorris ₁

Affiliation

Sensitive immunoassays are required for troponin, a low-abundance cardiac biomarker in blood. In contrast to conventional (analog) assays that measure the integrated signal of thousands of molecules, digital assays are based on counting individual biomarker molecules. Photon-upconversion nanoparticles (UCNP) are an excellent nanomaterial for labeling and detecting single biomarker molecules because their unique anti-Stokes emission avoids optical interference, and single nanoparticles can be reliably distinguished from the background signal. Here, the effect of the surface architecture and size of UCNP labels on the performance of upconversion-linked immunosorbent assays (ULISA) is critically assessed. The size, brightness, and surface architecture of UCNP labels are more important for measuring low troponin concentrations in human plasma than changing from an analog to a digital detection mode. Both detection modes result approximately in the same assay sensitivity, reaching a limit of detection (LOD) of 10 pg mL⁻¹ in plasma, which is in the range of troponin concentrations found in the blood of healthy individuals.

中文翻译：

光子上转换纳米颗粒的粒径和表面化学对心脏肌钙蛋白模拟和数字免疫测定的影响

肌钙蛋白是血液中一种低丰度的心脏生物标志物，需要进行灵敏的免疫测定。与测量数千个分子的综合信号的传统（模拟）分析相比，数字分析基于对单个生物标志物分子的计数。光子上转换纳米粒子 (UCNP) 是一种用于标记和检测单个生物标志物分子的出色纳米材料，因为它们独特的反斯托克斯发射避免了光学干扰，并且可以可靠地将单个纳米粒子与背景信号区分开来。在这里，UCNP 标签的表面结构和大小对上转换相关免疫吸附测定 (ULISA) 的性能的影响进行了严格评估。大小、亮度、UCNP 标记的表面结构和表面结构对于测量人血浆中的低肌钙蛋白浓度比从模拟检测模式更改为数字检测模式更重要。两种检测模式的检测灵敏度大致相同，检测限 (LOD) 为 10 pg mL^-1在血浆中，这是在健康个体血液中发现的肌钙蛋白浓度范围内。

更新日期：2021-09-22

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