Comparative genomics has become an important strategy for transferring genetic information from a well-characterized model species to one that is less described and can facilitate in functional gene mining and genomic evolution. Crassostrea angulata and Crassostrea gigas are closed related species that they can hybridize to produce fertile offspring, and C. gigas has accumulated a considerable number of genetic and genomic resources while C. angulata lags far behind. In the current study, 684 single nucleotide polymorphism (SNP) markers developed from C. gigas expressed sequence tags (ESTs) were used for transferability test in C. angulata, and 635 loci were successfully amplified. Then a genetic linkage map of C. angulata was for the first time constructed using these transferable SNP markers. A total of 988 transferable SNP markers were used for polymorphism screening in a mapping family, and 273 loci were distributed on the genetic map which was composed of 10 linkage groups and estimated 1040.29 cM in total length. The average spacing between markers was 3.96 cM. By comparing the shared EST-SNP marker locations on homologous linkage groups, comparative mapping analysis between the C. angulata genetic map and an integrated map of C. gigas revealed putative macro-collinearity in the two oyster genomes with little difference in markers order. These transferable SNP markers could be applied in genetic diversity and population structure studies, and the genetic map would be useful for quantitative trait loci mapping and genetic improvement through marker-assisted selection for C. angulata.

比较基因组学已成为将遗传信息从特征明确的模型物种转移到描述较少的物种的重要策略，可以促进功能基因挖掘和基因组进化。角线牡蛎和太平洋牡蛎是封闭相关的物种，它们能杂交产生育后代，并长牡蛎已经积累了相当数量的遗传和基因组资源，同时C.角线远远落后。在当前的研究中，从C. gigas表达序列标签 (EST)开发的 684 个单核苷酸多态性 (SNP) 标记用于C. angulata 的可转移性测试，并成功扩增了 635 个基因座。然后首次使用这些可转移的 SNP 标记构建了C. angulata的遗传连锁图。共有988个可转移SNP标记用于一个作图家族的多态性筛选，273个位点分布在由10个连锁群组成的遗传图谱上，估计总长度为1040.29 cM。标记之间的平均间距为 3.96 cM。通过比较同源连锁群上共享的 EST-SNP 标记位置，C. angulata遗传图谱和C. gigas整合图谱之间的比较作图分析揭示了两个牡蛎基因组中假定的宏观共线性，标记顺序几乎没有差异。这些可转移的 SNP 标记可用于遗传多样性和种群结构研究，该遗传图谱将有助于通过标记辅助选择对C. angulata进行数量性状基因座定位和遗传改良。