Bioprospecting beneficial endophytic bacterial communities associated with Rosmarinus officinalis for sustaining plant health and productivity,World Journal of Microbiology and Biotechnology

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Bioprospecting beneficial endophytic bacterial communities associated with Rosmarinus officinalis for sustaining plant health and productivity
World Journal of Microbiology and Biotechnology ( IF 4.0 ) Pub Date : 2021-07-15 , DOI: 10.1007/s11274-021-03101-7
Minakshi Sharma ₁ , Gaurav Sood ₂ , Anjali Chauhan ₂

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The present study aimed to isolate and identify root endophytic bacteria with multifunctional plant growth promoting (PGP) traits from medicinal plant Rosmarinus officinalis grown in the North-Western Himalayas. A total of 42 strains were isolated, exhibiting variable degrees of PGP traits, including phosphate solubilization (10–375 µg/mL), indole-3-acetic acid (6–66 µg/mL), siderophore (32.37%–301.48% SU) production and antifungal activity in terms of percent growth inhibition (% GI) against Fusarium oxysporum (44.44%–77.77% GI), Fusarium graminearum (48.88%–71.42% GI) and Rhizoctonia solani (44.44%–77.7% GI). The 16S rDNA sequencing results showed lineage of these strains to 15 genera viz., Aneurinibacillus, Bacillus, Beijerinckia, Cedecea, Ensifer, Enterobacter, Kosakonia, Lactobacillus, Lysobacter, Oxynema, Pseudomonas, Pantoea, Paenibacillus, Pseudoxanthomonas and Serratia. Out of 42 strains, 11 potential strains were selected for in vivo growth studies of R. officinalis. The results showed that the inoculation of Bacillus subtilis KU21, Pseudomonas aeruginosa SI12, and Cedecea lapagei KU14 significantly increased the physical growth parameters of plant over uninoculated control viz., number of lateral of branches (43.95%–46.39%), stem height (29.04%–38.57%), root length (32.31%–37.14%), shoot (34.76%–40.91%) and root biomass (62.89%–70.70%). Physiological characteristics such as total chlorophyll (30.41%–30.96%), phenol (14.43%–24.55%) and carotenoids (34.26%–39.87%) content, also showed a relative increase as compared to uninoculated control; furthermore, the macronutrients (NPK) contents of the plant as well as soil also showed an increase. The developed module may be recommended for sustainable production of R. officinalis in the North-Western Himalayan region without hampering the soil health and fertility.

Graphic abstract

中文翻译：

生物勘探与迷迭香相关的有益内生细菌群落，以维持植物健康和生产力

本研究旨在从喜马拉雅西北部种植的药用植物迷迭香中分离和鉴定具有多功能植物生长促进 (PGP) 特性的根内生细菌。共分离出 42 个菌株，表现出不同程度的 PGP 性状，包括磷酸盐增溶 (10–375 µg/mL)、吲哚-3-乙酸 (6–66 µg/mL)、铁载体 (32.37%–301.48% SU) ) 针对尖孢镰刀菌(44.44%–77.77% GI)、禾谷镰刀菌(48.88%–71.42% GI) 和立枯丝核菌(44.44%–77.7% GI) 的生长抑制百分比 (% GI) 的产生和抗真菌活性。16S rDNA 测序结果显示这些菌株的谱系为 15 个属，即Aneurinibacillus，芽孢杆菌，拜叶林克氏菌属，Cedecea，Ensifer，肠杆菌属，Kosakonia，乳杆菌，溶杆菌，Oxynema，假单胞菌，泛菌，芽孢杆菌，Pseudoxanthomonas和沙雷。在 42 个菌株中，选择了 11 个潜在菌株用于R. officinalis 的体内生长研究。结果表明，接种枯草芽孢杆菌KU21、铜绿假单胞菌SI12、Cedecea lapagei与未接种对照相比，KU14 显着增加了植物的物理生长参数，即侧枝数（43.95%–46.39%）、茎高（29.04%–38.57%）、根长（32.31%–37.14%）、枝条（ 34.76%–40.91%) 和根生物量 (62.89%–70.70%)。与未接种对照相比，总叶绿素（30.41%–30.96%）、苯酚（14.43%–24.55%）和类胡萝卜素（34.26%–39.87%）含量等生理特征也显示出相对增加；此外，植物和土壤的常量营养素 (NPK) 含量也显示出增加。该开发的模块，可推荐为可持续生产的R.巴戟在西北部的喜马拉雅地区，而不妨碍土壤健康和生育能力。

图形摘要

更新日期：2021-07-15

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