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Description and functional analysis of the transcriptome from malting barley
Genomics ( IF 3.4 ) Pub Date : 2021-07-15 , DOI: 10.1016/j.ygeno.2021.07.011
Marcus A Vinje 1 , Cynthia A Henson 2 , Stanley H Duke 3 , Carl H Simmons 1 , Khoa Le 4 , Evan Hall 4 , Cory D Hirsch 4
Affiliation  

The present study aimed to establish an early model of the malting barley transcriptome, which describes the expression of genes and their ontologies, identify the period during malting with the largest dynamic shift in gene expression for future investigation, and to determine the expression patterns of all starch degrading enzyme genes relevant to the malting and brewing industry. Large dynamic increases in gene expression occurred early in malting with differential expressed genes enriched for cell wall and starch hydrolases amongst many malting related categories. Twenty-five of forty starch degrading enzyme genes were differentially expressed in the malting barley transcriptome including eleven α-amylase genes, six β-amylase genes, three α-glucosidase genes, and all five starch debranching enzyme genes. Four new or novel α-amylase genes, one β-amylase gene (Bmy3), three α-glucosidase genes, and two isoamylase genes had appreciable expression that requires further exploration into their potential relevance to the malting and brewing industry.



中文翻译:

麦芽大麦转录组的描述和功能分析

本研究旨在建立麦芽大麦转录组的早期模型,该模型描述基因的表达及其本体,确定麦芽期间基因表达动态变化最大的时期,以供未来研究,并确定所有基因的表达模式。与麦芽和酿造工业相关的淀粉降解酶基因。基因表达的大幅动态增加发生在麦芽早期,差异表达基因富含细胞壁和淀粉许多与麦芽相关的类别中的水解酶。40个淀粉降解酶基因中有25个在麦芽大麦转录组中差异表达,包括11个α-淀粉酶基因、6个β-淀粉酶基因、3个α-葡萄糖苷酶基因和全部5个淀粉脱支酶基因。四个新的或新颖的 α-淀粉酶基因、一个 β-淀粉酶基因 ( Bmy3 )、三个 α-葡萄糖苷酶基因和两个异淀粉酶基因具有明显的表达,需要进一步探索它们与麦芽和酿造行业的潜在相关性。

更新日期:2021-07-30
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