ABSTRACT

Background and objective

Platelet-rich plasma (PRP) is a category of platelet concentrate that has been widely used as a therapeutic modality in musculoskeletal medicine. The present study was conducted to classify and code the non-activated platelet-rich plasma (nPRP) derived from New Zealand white rabbits for tissue engineering and other regenerative medicine applications.

Methods

PRP was prepared from the whole blood by double centrifugation protocol using a laboratory centrifuge. The prepared nPRP was characterized using the parameters such as platelet dose, the relative composition of platelets, WBC, and RBC. The production protocol was described using the parameters such as platelet enrichment factor, factor increase in WBC concentration, platelet capture efficiency, WBC-reducing efficiency, and RBC-reducing efficiency. The nPRP was also classified and coded using the most recent and universally accepted classification and coding systems.

Results

The non-activated leukocyte-poor red cell-rich PRP had an average platelet count of 1875.75 × 10⁹/L, which is higher than the basal platelet concentration in the whole blood. Furthermore, the protocol used in this study has a mean platelet capture efficiency of 47.43 ± 6.42%.

Conclusion

The protocol described in this study can be used to prepare non-activated leukocyte-poor red cell-rich PRP (Red-PRP IC1) from rabbits that can be coded as 318–00-00.

中文翻译：

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用于组织工程和再生医学应用的新西兰白兔富含血小板血浆的分类和编码

摘要

背景和目标

富血小板血浆 (PRP) 是一类血小板浓缩物，已广泛用作肌肉骨骼医学的治疗方式。本研究旨在对来自新西兰白兔的非活化富血小板血浆 (nPRP) 进行分类和编码，用于组织工程和其他再生医学应用。

方法

PRP 是通过使用实验室离心机的双重离心方案从全血中制备的。使用血小板剂量、血小板相对组成、WBC 和 RBC 等参数对制备的 nPRP 进行表征。使用血小板富集因子、WBC 浓度增加因子、血小板捕获效率、WBC 减少效率和 RBC 减少效率等参数描述了生产方案。nPRP 也使用最新和普遍接受的分类和编码系统进行分类和编码。

结果

未活化的贫白细胞富红细胞PRP的平均血小板计数为1875.75×10 ⁹ /L，高于全血基础血小板浓度。此外，本研究中使用的协议的平均血小板捕获效率为 47.43 ± 6.42%。

结论

本研究中描述的协议可用于从可编码为 318-00-00 的兔子中制备非活化白细胞贫红细胞丰富的 PRP (Red-PRP IC1)。