Viscous camelid ejaculates present problems for sperm handling and sperm preservation. In the present study, a technique that had been used for dromedary camel semen was tested with alpaca semen. Ejaculates (n=9) were collected by artificial vagina at San Marcos University, Lima, and were liquefied by gentle pipetting in tris-citrate-fructose. Half of the sample was prepared by Single Layer Centrifugation (SLC) through a colloid; the other half was centrifuged without colloid as a control. Each control and SLC sample was then split into two parts; one part was stored cooled for 24 h at 5 °C and the other part was frozen, resulting in 4 treatments for each ejaculate. All samples were evaluated for sperm motility, hypoosmotic swelling test (HOST), plasma membrane integrity, and morphology, immediately after centrifugation and again after storage Total motility and plasma membrane integrity were greater in samples prepared by SLC than controls (motility 72±13% vs. 57±7%; plasma membrane integrity 63±13% vs. 54±8%, for SLC and controls respectively). Normal morphology and HOST were not different between treatments (65±13 vs. 61±13% and 42±6 vs. 39±10%, for SLC and controls respectively). After 24 h cooled storage, motility and plasma membrane integrity were greater for SLC samples (motility: 51±16 vs. 34±15%; p<0.001; membrane integrity: 51±15 vs. 40±18%; P < 0.05 for SLC and controls, respectively); HOST (40±14 vs. 34±11%) and normal morphology (67±13 vs. 63±14%) were not different between treatments. Sperm quality decreased considerably after cryopreservation (P<0.001 for all parameters); however, motility (P<0.01), plasma membrane integrity (P<0.05) and morphology (P<0.05) were higher for SLC than for controls. These results indicate that alpaca spermatozoa can be extracted from semen using a combination of pipetting and SLC, potentially with a beneficial effect on sperm quality. Samples could be stored cooled for 24 h, retaining better motility than controls; motility and plasma membrane integrity were greater in SLC samples than controls after freezing and thawing but the freezing protocol requires improvement.

粘性骆驼科动物精液存在精子处理和精子保存问题。在本研究中，一种曾用于单峰骆驼精液的技术用羊驼精液进行了测试。精液 (n=9) 由利马圣马科斯大学的人工阴道收集，并通过轻轻移液在三柠檬酸果糖中液化。一半的样品通过胶体通过单层离心 (SLC) 制备；另一半在没有胶体的情况下离心作为对照。然后将每个对照和 SLC 样品分成两部分；一份在 5°C 下冷却 24 小时，另一份冷冻，每次射精进行 4 次处理。对所有样品的精子活力、低渗膨胀试验 (HOST)、质膜完整性和形态进行了评估，离心后立即和储存后再次使用 SLC 制备的样品的总运动性和质膜完整性高于对照（运动性 72±13% 与 57±7%；质膜完整性 63±13% 与 54±8%，对于SLC 和控制分别）。正常形态和宿主在治疗之间没有差异（SLC 和对照分别为 65±13 对 61±13% 和 42±6 对 39±10%）。冷藏 24 小时后，SLC 样品的运动性和质膜完整性更高（运动性：51±16 对 34±15%；p<0.001；膜完整性：51±15 对 40±18%；SLC 和对照分别为 61±13% 和 42±6 与 39±10%）。冷藏 24 小时后，SLC 样品的运动性和质膜完整性更高（运动性：51±16 对 34±15%；p<0.001；膜完整性：51±15 对 40±18%；SLC 和对照分别为 61±13% 和 42±6 与 39±10%）。冷藏 24 小时后，SLC 样品的运动性和质膜完整性更高（运动性：51±16 对 34±15%；p<0.001；膜完整性：51±15 对 40±18%；SLC 和对照的P < 0.05，分别）；HOST (40±14 vs. 34±11%) 和正常形态 (67±13 vs. 63±14%) 在治疗之间没有差异。冷冻保存后精子质量显着下降（所有参数P <0.001）；然而，运动性（P <0.01），质膜完整性（P <0.05）和形态（P<0.05) 的 SLC 高于对照组。这些结果表明，可以使用移液和 SLC 的组合从精液中提取羊驼精子，这可能对精子质量产生有益影响。样品可以冷藏保存 24 小时，比对照保持更好的运动性；在冷冻和解冻后，SLC 样品中的运动性和质膜完整性比对照更大，但冷冻方案需要改进。