Abstract

The article discusses the effect of copper oxide (CuO) and silver (Ag) nanoparticles on expression of genes asr3, icdh, PR-1, PR-10, DREB2, used as markers of the development of stress tolerance, in poplar in vitro clones of the “POK” variety under conditions of artificial infection with the filamentous ascomycete Alternaria alternata. The exposure to the phytopathogen activates the asr3, icdh, PR-1, PR-10, DREB2 genes in microclones on a medium without nanoparticles, which indicates development of adaptation mechanisms in them. The expression indicators of stress tolerance marker genes in in vitro clones on a medium with CuO and Ag nanoparticles were reduced or did not exceed the values obtained for plants on a medium without nanoparticles. The observed suppression of the stress response under cultivation with CuO and Ag may be governed by the inhibitory effect of nanoparticles on the development of Alternaria alternata, as well as their influence on the development processes of poplar in vitro clones of the POK variety, which is associated with the peculiarities of the plant genotype and the concentration of nanoparticles.

中文翻译：

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氧化铜和纳米银颗粒对杨树体外无性系交链孢菌耐受性发展的影响

摘要

这篇文章讨论了氧化铜 (CuO) 和银 (Ag) 纳米粒子对杨树体外无性系中用作胁迫耐受性发展标记的基因asr3 , icdh , PR-1 , PR-10 , DREB2 表达的影响“POK”品种在人工感染丝状子囊菌Alternaria alternata 的条件下。暴露于植物病原体会激活asr3、icdh、PR-1、PR-10、DREB2在没有纳米颗粒的介质上的微克隆中的基因，这表明它们的适应机制的发展。在含有CuO 和Ag 纳米颗粒的培养基上的体外克隆中，胁迫耐受标记基因的表达指标降低或不超过在不含纳米颗粒的培养基上的植物获得的值。观察到的 CuO 和 Ag 对胁迫响应的抑制可能是由纳米颗粒对链格孢发育的抑制作用以及它们对 POK 品种杨树体外无性系发育过程的影响所控制的。与植物基因型的特性和纳米颗粒的浓度有关。