In recent years, light-sheet fluorescence microscopy (LSFM) has found a broad application for imaging of diverse biological samples, ranging from sub-cellular structures to whole animals, both in-vivo and ex-vivo, owing to its many advantages relative to point-scanning methods. By providing the selective illumination of sample single planes, LSFM achieves an intrinsic optical sectioning and direct 2D image acquisition, with low out-of-focus fluorescence background, sample photo-damage and photo-bleaching. On the other hand, such an illumination scheme is prone to light absorption or scattering effects, which lead to uneven illumination and striping artifacts in the images, oriented along the light sheet propagation direction. Several methods have been developed to address this issue, ranging from fully optical solutions to entirely digital post-processing approaches. In this work, we present them, outlining their advantages, performance and limitations.

近年来，光片荧光显微镜 (LSFM) 已发现广泛应用于各种生物样品的成像，从亚细胞结构到整个动物，无论是体内还是离体，由于其相对于点扫描方法。通过提供样品单平面的选择性照明，LSFM 实现了固有的光学切片和直接 2D 图像采集，具有低离焦荧光背景、样品光损伤和光漂白。另一方面，这种照明方案容易产生光吸收或散射效应，从而导致图像中的照明不均匀和条纹伪影，沿光片传播方向取向。已经开发了几种方法来解决这个问题，从全光学解决方案到全数字后处理方法。在这项工作中，我们介绍了它们，概述了它们的优点、性能和局限性。