This study investigated the effects of dietary tea tree oil (TTO) supplementation and feeding patterns on the growth and nonspecific immunity of Macrobrachium rosenbergii. Prawns were randomly assigned into four patterns. Patterns 1 (P1) and 4 (P4): prawns were fed with a basal diet and basal diet supplemented with 100 mg/kg of TTO, respectively; Patterns 2 (P2) and 3 (P3): prawns were fed with the basal diet supplemented with 100 mg/kg of TTO at 1- and 2-week intervals, respectively. A significant increase in growth performance was observed in the P3 and P4 compared with P1. The activities of antioxidant enzyme were significantly elevated in the P3 and P4 compared with the P1. The concentration of haemolymph malondialdehyde was significantly lower in the P3 and P4 than the P1. The haemolymph level of IL-1 and TNF-α was significantly lower in the P3 than the P4. The expression of toll and dorsal was significantly lower in the P3 than the P1. However, the expression of peroxiredoxin-5 was significantly higher in the P3 than the P1. Overall, these findings indicated that feeding 100 mg/kg of TTO at 2-week intervals significantly improved the growth performance and nonspecific immunity of M. rosenbergii.

中文翻译：

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日粮茶树（互叶白千层）油和摄食模式对罗氏沼虾（Macrobrachium rosenbergii）畜牧业性能和非特异性免疫反应的影响

本研究调查了膳食茶树油 (TTO) 补充剂和喂养模式对罗氏沼虾生长和非特异性免疫的影响. 虾被随机分配成四种模式。模式 1 (P1) 和 4 (P4)：对虾分别饲喂基础日粮和添加 100 mg/kg TTO 的基础日粮；模式 2 (P2) 和 3 (P3)：分别以 1 周和 2 周的间隔喂食对虾添加 100 mg/kg TTO 的基础日粮。与 P1 相比，P3 和 P4 的生长性能显着提高。与P1相比，P3和P4的抗氧化酶活性显着升高。P3和P4的血淋巴丙二醛浓度明显低于P1。P3中IL-1和TNF-α的血淋巴水平显着低于P4。toll和背的表达P3明显低于P1。然而， P3中过氧化还原蛋白5的表达明显高于P1。总体而言，这些研究结果表明，每隔 2 周饲喂 100 mg/kg TTO 可显着改善罗氏沼虾的生长性能和非特异性免疫。