Osteosarcoma is a quickly developing, malignant cancer of the bone, which is associated with a bad prognosis. In osteosarcoma, hypoxia promotes the malignant phenotype, which results in a cascade of immunosuppressive processes, poor prognosis, and a high risk of metastasis. Nonetheless, additional methodologies for the study of hyperoxia in the tumor microenvironment also need more analysis. We obtained 88 children patients with osteosarcoma from the Therapeutically Applicable Research to Generate Effective Treatment (TARGET) database and 53 children patients with RNA sequence and clinicopathological data from the Gene Expression Omnibus (GEO). We developed a four-gene signature related to hypoxia to reflect the immune microenvironment in osteosarcoma that predicts survival. A high-risk score indicated a poor prognosis and immunosuppressive microenvironment. The presence of the four-gene signature related to hypoxia was correlated with clinical and molecular features and was an important prognostic predictor for pediatric osteosarcoma patients. In summary, we established and validated a four-gene signature related to hypoxia to forecast recovery and presented an independent prognostic predictor representing overall immune response strength within the osteosarcoma microenvironment.

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基于缺氧基因的特征预测儿童骨肉瘤的存活和影响肿瘤免疫微环境

骨肉瘤是一种快速发展的恶性骨癌，预后不良。在骨肉瘤中，缺氧会促进恶性表型，导致一系列免疫抑制过程、预后不良和转移风险高。尽管如此，研究肿瘤微环境中高氧的其他方法也需要更多的分析。我们从 Therapeutical Applicable Research to Generative Effective Treatment (TARGET) 数据库中获取了 88 名儿童骨肉瘤患者，并从 Gene Expression Omnibus (GEO) 中获取了 53 名儿童患者的 RNA 序列和临床病理数据。我们开发了一种与缺氧相关的四基因特征，以反映骨肉瘤中预测存活的免疫微环境。高风险评分表明预后不良和免疫抑制微环境。与缺氧相关的四基因特征的存在与临床和分子特征相关，是儿科骨肉瘤患者的重要预后预测因子。总之，我们建立并验证了与缺氧相关的四基因特征以预测恢复，并提出了代表骨肉瘤微环境中整体免疫反应强度的独立预后预测因子。