Introduction. The presence of Candida biofilms in medical devices is a concerning and important clinical issue for haemodialysis patients who require constant use of prosthetic fistulae and catheters. Hypothesis/Gap Statement. This prolonged use increases the risk of candidaemia due to biofilm formation. PH151 and clioquinol are 8-hydroxyquinoline derivatives that have been studied by our group and showed interesting anti-Candida activity. Aim. This study evaluated the biofilm formation capacity of Candida species on polytetrafluoroethylene (PTFE) and polyurethane (PUR) and investigated the synergistic effects between the compounds PH151 and clioquinol and fluconazole, amphotericin B and caspofungin against biofilm cells removed from those materials. Further, the synergistic combination was evaluated in terms of preventing biofilm formation on PTFE and PUR discs. Methodology. Susceptibility testing was performed for planktonic and biofilm cells using the broth microdilution method. The checkerboard method and the time–kill assay were used to evaluate the interactions between antifungal agents. Antibiofilm activity on PTFE and PUR materials was assessed to quantify the prevention of biofilm formation. Results. Candida albicans, Candida glabrata and Candida tropicalis showed ability to form biofilms on both materials. By contrast, Candida parapsilosis did not demonstrate this ability. Synergistic interaction was observed when PH151 was combined with fluconazole in 77.8 % of isolates and this treatment was shown to be concentration- and time-dependent. On the other hand, indifferent interactions were predominantly observed with the other combinations. A reduction in biofilm formation on PUR material of more than 50 % was observed when using PH151 combined with fluconazole. Conclusion. PH151 demonstrated potential as a local treatment for use in a combination therapy approach against Candida biofilm formation on haemodialysis devices.

中文翻译：

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8-羟基喹啉-5-(N-4-氯苯基)磺酰胺和氟康唑组合作为血液透析装置中念珠菌生物膜的预防策略

介绍。对于需要持续使用假体瘘管和导管的血液透析患者来说，医疗器械中念珠菌生物膜的存在是一个令人担忧的重要临床问题。假设/差距陈述。由于生物膜的形成，这种长期使用会增加念珠菌血症的风险。PH151 和氯碘羟喹是我们小组研究的 8-羟基喹啉衍生物，并显示出有趣的抗念珠菌活性。目标。本研究评估了念珠菌的生物膜形成能力聚四氟乙烯 (PTFE) 和聚氨酯 (PUR) 上的物种，并研究了化合物 PH151 与氯碘羟喹和氟康唑、两性霉素 B 和卡泊芬净对从这些材料中去除的生物膜细胞的协同作用。此外，在防止 PTFE 和 PUR 盘上形成生物膜方面对协同组合进行了评估。方法。使用肉汤微量稀释法对浮游生物和生物膜细胞进行敏感性测试。棋盘法和时间-杀灭试验用于评估抗真菌剂之间的相互作用。对 PTFE 和 PUR 材料的抗生物膜活性进行了评估，以量化对生物膜形成的预防。结果。 白色念珠菌、光滑念珠菌和热带念珠菌显示出在两种材料上形成生物膜的能力。相比之下，近平滑念珠菌没有表现出这种能力。在 77.8% 的分离株中观察到 PH151 与氟康唑组合时的协同相互作用，并且这种处理显示出浓度和时间依赖性。另一方面，在其他组合中主要观察到无差异的相互作用。当使用 PH151 与氟康唑组合时，观察到 PUR 材料上的生物膜形成减少了 50% 以上。结论。PH151 显示出作为局部治疗的潜力，可用于针对血液透析装置上念珠菌生物膜形成的联合治疗方法。