Long noncoding RNAs (lncRNAs) have been reported to be involved in various cellular processes and to participate in a variety of human diseases. Recently, increasing studies have reported that lncRNAs are related to many reproductive diseases, such as pathogenesis of recurrent pregnancy loss (RPL), preeclampsia (PE) and gestational diabetes mellitus (GDM). In this study, we aimed to investigate the effect of LINC01088 in trophoblast cells and its potential role in pathogenesis of RPL. LINC01088 was found to be upregulated in first-trimester chorionic villi tissues from RPL patients. Increased LINC01088 repressed proliferation, migration and invasion of trophoblast cells, and promoted apoptosis of trophoblast cells. Further exploration indicated that LINC01088 decreased the production of nitric oxide (NO) by binding and increasing Arginase-1 and decreasing eNOS protein levels. Importantly, JNK and p38 MAPK-signaling pathways were active after overexpression of LINC01088. In conclusion, our studies demonstrated that LINC01088 plays an important role in the pathogenesis of RPL, and is a potential therapeutic target for the treatment of RPL.

中文翻译：

---

LncRNA LINC01088 抑制滋养层细胞的功能，激活 MAPK 信号通路并与复发性流产相关


据报道，长非编码RNA（lncRNA）参与多种细胞过程并参与多种人类疾病。近年来，越来越多的研究报道lncRNA与许多生殖疾病有关，例如复发性妊娠丢失（RPL）、先兆子痫（PE）和妊娠期糖尿病（GDM）的发病机制。在本研究中，我们旨在研究 LINC01088 对滋养层细胞的影响及其在 RPL 发病机制中的潜在作用。研究发现 LINC01088 在 RPL 患者的孕早期绒毛膜组织中表达上调。 LINC01088的增加抑制滋养层细胞的增殖、迁移和侵袭，并促进滋养层细胞的凋亡。进一步的研究表明，LINC01088 通过结合和增加 Arginase-1 以及降低 eNOS 蛋白水平来减少一氧化氮 (NO) 的产生。重要的是，JNK 和 p38 MAPK 信号通路在 LINC01088 过表达后活跃。总之，我们的研究表明LINC01088在RPL的发病机制中发挥着重要作用，是治疗RPL的潜在治疗靶点。