Human SW872 preadipocyte conversion to mature adipocytes is associated with time-dependent changes in differentiation markers' expression and with morphological changes accompanied by the accumulation of lipid droplets (LDs) as well as by increased mitochondriogenesis and mitochondrial membrane potential. Under identical conditions, the formation of reactive oxygen species (ROS) revealed with a general probe was significant at days 3 and 10 of differentiation and bearly detectable at day 6. NADPH oxidase (NOX)-2 activity determined with an immunocytochemical approach followed a very similar pattern. There was no evidence of mitochondrial ROS (mROS), as detected with a selective fluorescence probe, at days 3 and 6, possibly due to the triggering of the Nrf-2 antioxidant response. mROS were instead clearly detected at day 10, concomitantly with the accumulation of very large LDs, oxidation of both cardiolipin and thioredoxin 2, and decreased mitochondrial glutathione. In conclusion, the morphological and biochemical changes of differentiating SW872 cells are accompanied by the discontinuous formation of ROS derived from NOX-2, increasingly implicated in adipogenesis and adipose tissue dysfunction. In addition, mROS formation was significant only in the late phase of differentiation and was associated with mitochondrial dysfunction.

中文翻译：

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人 SW872 细胞成脂分化过程中形态和生化变化与不同活性氧形成机制募集的时间相关性

人类 SW872 前脂肪细胞向成熟脂肪细胞的转化与分化标志物表达的时间依赖性变化以及伴随脂滴 (LD) 积累以及线粒体生成和线粒体膜电位增加的形态变化有关。在相同条件下，一般探针显示的活性氧 (ROS) 的形成在分化的第 3 天和第 10 天是显着的，并且在第 6 天几乎可以检测到。用免疫细胞化学方法测定的 NADPH 氧化酶 (NOX)-2 活性遵循非常类似的模式。在第 3 天和第 6 天，用选择性荧光探针检测到没有线粒体 ROS (mROS) 的证据，这可能是由于触发了 Nrf-2 抗氧化反应。相反，在第 10 天清楚地检测到 mROS，伴随着非常大的 LD 的积累，心磷脂和硫氧还蛋白 2 的氧化，以及线粒体谷胱甘肽的减少。总之，分化的 SW872 细胞的形态和生化变化伴随着来自 NOX-2 的 ROS 的不连续形成，越来越多地涉及脂肪生成和脂肪组织功能障碍。此外，mROS 的形成仅在分化后期才显着，并且与线粒体功能障碍有关。越来越多地涉及脂肪生成和脂肪组织功能障碍。此外，mROS 的形成仅在分化后期才显着，并且与线粒体功能障碍有关。越来越多地涉及脂肪生成和脂肪组织功能障碍。此外，mROS 的形成仅在分化后期才显着，并且与线粒体功能障碍有关。